|
| Status |
Public on Sep 05, 2008 |
| Title |
K9 vs DBY8268 #2 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
DBY8268 grown in YPD medium
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
Strain DBY8268
|
| Treatment protocol |
No treatment
|
| Growth protocol |
Unless otherwise noted, cells were grown 3 doublings in YPD at 30C to OD600 ~0.5
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Hot-phenol RNA extraction as described in Gasch AP Methods in Enzymology 350: 393-414
|
| Label |
Oyster 550 cyanine dye
|
| Label protocol |
Indirect labeling using amino-allyl-dUTP and cyanine dyes from Flownamics (Madison, Wi) as described in Gasch AP Methods in Enzymology 350: 393-414
|
| |
|
| Channel 2 |
| Source name |
K9 grown in YPD medium
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
Strain K9
|
| Treatment protocol |
No treatment
|
| Growth protocol |
Unless otherwise noted, cells were grown 3 doublings in YPD at 30C to OD600 ~0.5
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Hot-phenol RNA extraction as described in Gasch AP Methods in Enzymology 350: 393-414
|
| Label |
Oyster 650 cyanine dye
|
| Label protocol |
Indirect labeling using amino-allyl-dUTP and cyanine dyes from Flownamics (Madison, Wi) as described in Gasch AP Methods in Enzymology 350: 393-414
|
| |
|
| |
| Hybridization protocol |
As indicated in Gasch AP Methods in Enzymology 350: 393-414
|
| Scan protocol |
Arrays were scanned on an Axon 4000B scanner
|
| Description |
Comparison of Strain DBY8268 to Strain K9
yG5-51b
|
| Data processing |
Data were extracted using GenePix 6.0. Data were normalized by regional mean-centering as described by Lyne et al. 2003 BMC Genomics
|
| |
|
| Submission date |
Jan 24, 2008 |
| Last update date |
Aug 20, 2009 |
| Contact name |
Audrey P. Gasch |
| E-mail(s) |
agasch@wisc.edu
|
| Phone |
(608)265-0859
|
| Fax |
(608)262-2976
|
| Organization name |
University of Wisconsin Madison
|
| Department |
Laboratory of Genetics
|
| Lab |
Gasch Lab
|
| Street address |
425-g Henry Mall
|
| City |
Madison |
| State/province |
WI |
| ZIP/Postal code |
53706 |
| Country |
USA |
| |
|
| Platform ID |
GPL5915 |
| Series (2) |
| GSE10268 |
Variations in stress sensitivity and genomic expression in diverse S. cerevisiae strains (gene expression) |
| GSE10269 |
Variations in stress sensitivity and genomic expression in diverse S. cerevisiae strains |
|
