|
| Status |
Public on Sep 06, 2017 |
| Title |
MDAMB231_4175_siPGC1alpha_1 |
| Sample type |
RNA |
| |
|
| Source name |
4175 cells, siPGC1alpha
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: MDA-MB-231 lung variant (4175)
|
| Treatment protocol |
4175 cells were treated with sicontrol or sipgc1alpha as described here, materials and methods
|
| Growth protocol |
4175 cells were cultured and grown as described in this paper, materials and methods
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA from 4175 cells were isolated in tissue culture using an Aurum Total RNA Mini Kit.
|
| Label |
Aurum Total RNA Mini Kit
|
| Label protocol |
Sense-strand cDNA was synthesized from 10 ng of total RNA and fragmentation and labeling were performed to produce ds DNA with the GeneChip® Pico Labeling Kit according to manufacturer’s instructions (Applied Biosystems)
|
| |
|
| Hybridization protocol |
After fragmentation and labeling, 4 µg DNA target was hybridized on GeneChip® Human Clariom™ S (ThermoFisher)and incubated at 450C in the Genechip® Hybridization oven 640 (ThermoFisher) for 17 hours at 60 rpm
|
| Scan protocol |
The microarrays were finally scanned on a GeneChip® scanner 3000 (ThermoFisher).
|
| Description |
cell culture
expression data from human lung metastatic 4175 cells treated with siPGC1alpha
|
| Data processing |
Bioconductor. The file 'normalized_data_allcontrols.txt' contains data for all probe sets including controls. The sample table does not include data for AFFX controls, *RPTR controls or probe sets named with 230*.
|
| |
|
| Submission date |
Jun 01, 2017 |
| Last update date |
Jan 23, 2018 |
| Contact name |
Julie St-Pierre |
| E-mail(s) |
julie.st-pierre@mcgill.ca
|
| Organization name |
McGill University
|
| Department |
Department of Biochemistry
|
| Street address |
3655 Promenade Sir William Osler
|
| City |
Montreal |
| State/province |
Quebec |
| ZIP/Postal code |
H3G 1Y6 |
| Country |
Canada |
| |
|
| Platform ID |
GPL23159 |
| Series (2) |
| GSE99554 |
Expression data from a human lung metastatic cell line treated with siPGC1alpha or siControl |
| GSE99557 |
Expression data from a lung metastatic cell line or metastatic explants in mouse and human |
|
