|
Status |
Public on Jun 23, 2017 |
Title |
Lesional Skin Biopsy [45583004501_D15] |
Sample type |
RNA |
|
|
Source name |
Lesional Skin Biopsy
|
Organism |
Homo sapiens |
Characteristics |
tissue: skin diagnosis: Discoid Lupus Erythematosus skin lesion status: Lesional sbj: 45583004501 visit name: D15 dose: 180 mg sequence: AMG811 on Day 1 followed by Placebo (D85)
|
Treatment protocol |
The study was designed as a phase I randomized, double-blind, placebo-controlled crossover study of the pharmacodynamics, safety, and clinical efficacy of AMG 811 in patients with DLE. Patients received a single subcutaneous dose of AMG 811 (180 mg) or placebo. The patients in sequence 1 received AMG 811 followed by placebo, while those in sequence 2 received placebo followed by AMG 811.
|
Extracted molecule |
total RNA |
Extraction protocol |
Frozen skin samples were disrupted with a Multi-Sample Bio Pulverizer (Research Products International) and homogenized with a TissueRuptor (Qiagen). Total RNA was isolated using the mirVana Micro-RNA Isolation kit (Applied Biosystems), modified to include on-column DNase treatment with RNase-free DNase (Qiagen).
|
Label |
biotin
|
Label protocol |
Labeled complementary DNA was generated using a NuGEN Ovation kit (NuGen Technologies) according to the manufacturer's instructions.
|
|
|
Hybridization protocol |
Labeled samples were hybridized to GeneChip HT_HG-U133+PM microarrays.
|
Scan protocol |
Hybridizaed microarrays were scanned using a GeneTitan instrument (Affymetrix)
|
Description |
Sample name: 48
|
Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings.
|
|
|
Submission date |
Jun 15, 2017 |
Last update date |
Jan 23, 2018 |
Contact name |
Dan Baker |
E-mail(s) |
bakerd@amgen.com
|
Phone |
805-447-1000
|
Organization name |
Amgen Inc.
|
Department |
Molecular Sciences
|
Street address |
1 Amgen Center Drive
|
City |
Thousand Oaks |
State/province |
CA |
ZIP/Postal code |
91320 |
Country |
USA |
|
|
Platform ID |
GPL13158 |
Series (2) |
GSE100093 |
Pharmacodynamics, Safety, and Clinical Efficacy of AMG 811, a Human Anti-Interferon-γ Antibody, in Patients With Discoid Lupus Erythematosus [skin] |
GSE100431 |
Pharmacodynamics, Safety, and Clinical Efficacy of AMG 811, a Human Anti-Interferon-γ Antibody, in Patients With Discoid Lupus Erythematosus |
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