|
Status |
Public on Jun 23, 2017 |
Title |
blood [91166005057_D85.6] |
Sample type |
RNA |
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Source name |
Blood
|
Organism |
Homo sapiens |
Characteristics |
tissue: blood diagnosis: Discoid Lupus Erythematosus sbj: 91166005057 visit name: D85.6 sequence: Placebo on Day 1 followed by AMG811 (D85)
|
Treatment protocol |
The study was designed as a phase I randomized, double-blind, placebo-controlled crossover study of the pharmacodynamics, safety, and clinical efficacy of AMG 811 in patients with DLE. Patients received a single subcutaneous dose of AMG 811 (180 mg) or placebo. The patients in sequence 1 received AMG 811 followed by placebo, while those in sequence 2 received placebo followed by AMG 811.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was isolated from PAXgene whole blood tubes (BD Cat # 762165) using the PAXgene RNA Kit. (Qiagen Cat # 762164) on the Qiacube automated sample prep system
|
Label |
Cy3
|
Label protocol |
Samples were labeled using the Agilent Low RNA Input Linear Amplification Kit PLUS, One-Color (Agilent Cat # 5188-5339) per manufacturer’s instructions.
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|
|
Hybridization protocol |
Overnight hybridizations at 65C per manufacturer’s instructions
|
Scan protocol |
All arrays were scanned on the Agilent Surescaner Scanner (Agilent p/n G2600D). Agilent Scan Control Software version 9.1.5.1 was used for array scanning. Array files were processed using Agilent Feature Extraction software 11.0.1.1.
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Data processing |
The scanned images were analyzed using default parameters (protocol GE1-v1_91 and Grid: 026822_D_F_20100209) to obtain background subtracted and spatially detrended Processed Signal intensities. Features flagged in Feature Extraction as Feature Non-uniform outliers were excluded.
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|
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Submission date |
Jun 23, 2017 |
Last update date |
Jan 23, 2018 |
Contact name |
Dan Baker |
E-mail(s) |
bakerd@amgen.com
|
Phone |
805-447-1000
|
Organization name |
Amgen Inc.
|
Department |
Molecular Sciences
|
Street address |
1 Amgen Center Drive
|
City |
Thousand Oaks |
State/province |
CA |
ZIP/Postal code |
91320 |
Country |
USA |
|
|
Platform ID |
GPL23613 |
Series (2) |
GSE100430 |
Pharmacodynamics, Safety, and Clinical Efficacy of AMG 811, a Human Anti-Interferon-γ Antibody, in Patients With Discoid Lupus Erythematosus [blood] |
GSE100431 |
Pharmacodynamics, Safety, and Clinical Efficacy of AMG 811, a Human Anti-Interferon-γ Antibody, in Patients With Discoid Lupus Erythematosus |
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