Cell lines RKO, SW48, HT55, SW948, SW1463, CL40, LS180, CAR1, HUTU80 and OUMS23 were cultured in Dulbecco's modified Eagle's medium (DMEM)/F-12 medium with Ll-glutamine, 15 mM HEPES (Thermo-Fisher Scientific, Bleiswijk, The Netherlands) with added fetal bovine serum (Lonza, Breda, The Netherlands) and penicillin and streptomycin. HCT-116, KM12, RCM-1, SNU-C1, LS-513, COLO-320-HSR, MDST8 and NCI-H716 were cultured in RPMI 1640 with Ll-glutamine, 25 mM HEPES (Thermo-Fisher Scientific) added fetal bovine serum, penicillin and streptomycin, 1% Dd-glucose solution plus (Sigma-Aldrich, Zwijndrecht, The Netherlands) and 100 μM sodium pyruvate (Life Technologies, Bleiswijk, The Netherlands). All cultures were maintained in humidified 37°C 5% CO2 incubators and cells were regularly tested for mycoplasma infection.
Extracted molecule
total RNA
Extraction protocol
Total RNA was extracted using the AllPrep DNA/RNA/miRNA Universal Kit from Qiagen.
Label
biotin
Label protocol
Labeling was performed according to the standard protocols of the Cologne Center for Genomics (CCG), University of Cologne, Germany.
Hybridization protocol
Hybridization was performed according to the standard protocols of the Cologne Center for Genomics (CCG), University of Cologne, Germany.
Scan protocol
Samples were scanned using the Gene Titan Affymetrix instrument according to the standard protocols of the Cologne Center for Genomics (CCG), University of Cologne, Germany.
Data processing
Data were normalized by RMA using the Affy R package
Submission date
Jun 26, 2017
Last update date
Jan 23, 2018
Contact name
Sander Roeland van Hooff
Organization name
AUMC
Department
Center for Experimental and Molecular Medicine (CEMM),
Lab
Laboratory for Experimental Oncology and Radiobiology (LEXOR)
