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| Status |
Public on Mar 27, 2019 |
| Title |
00018 |
| Sample type |
SRA |
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| Source name |
Mononuclear cells, healthy control
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| Organism |
Homo sapiens |
| Characteristics |
disease status: Healthy
tissue: Peripheral blood
gender: Male
age: 42
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| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA (1 μg) was used for removing the rRNAs using Ribo-Zero rRNA Removal Kits (Illumina, San Diego, CA, USA) following the manufacturer's instructions.
RNA libraries were constructed by using rRNA-depleted RNAs with TruSeq Stranded Total RNA Library Prep Kit (Illumina, San Diego, CA, USA) according to the manufacturer’s instructions. Libraries were controlled for quality and quantified using the BioAnalyzer 2100 system (Agilent Technologies, Inc., USA).
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 4000 |
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| Description |
Processed data file: mRNA Expression Profiling.xlsx
Processed data file: LncRNA Expression Profiling.xlsx
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| Data processing |
Basecalls performed using CASAVA version 1.8.
RNA-seq reads were mapped to human genome (UCSC hg19) by hisat2 (v2.04) software with default parameters.
Cuffdiff2 software (v2.2.1) was used to perform differentially expressed gene analysis.
Genome_build: hg19 (GRCh37)
Supplementary_files_format_and_content: *xlsx: Excel files include FPKMs.
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| Submission date |
Jul 18, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Huang Guangyong |
| E-mail(s) |
hgygzrzy@163.com
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| Phone |
15275810843
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| Organization name |
Shandong University
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| Street address |
Cultural West Road,No.44
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| City |
Ji'nan |
| ZIP/Postal code |
250000 |
| Country |
China |
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| Platform ID |
GPL20301 |
| Series (1) |
| GSE101585 |
Aberrant expression profile of lncRNA and mRNA in dilated cardiomyopathy by RNA-sequence |
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| Relations |
| BioSample |
SAMN07362140 |
| SRA |
SRX3014304 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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