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Status
Public on Nov 05, 2004
Title
INS-1 cell line (FLP-In T-REx clone #5-3.14)
Sample type
RNA
Source name
INS-1 cell line, clone #5-3.14
Organism
Rattus norvegicus
Extracted molecule
total RNA
Description
Starting with the rat insulinoma cell line INS-1, clones were generated that contain a FRT site for site specific integration of specific genes (INS-1 Flp-In T-REx). In addition, the clones contain the tetracycline repressor allowing tetracycline (tet) induction of the introduced gene. By comparing the gene expression profiles of uninduced and tet-induced cells, target genes of introduced transcription factor can be identified. This entry corresponds to the RNA isolated from the INS-1 cell line #5-3.14 Keywords = INS-1 Keywords = b-cell Keywords = Flp-In T-Rex Keywords = tetracycline Keywords = FRT
Submission date
Jul 22, 2004
Last update date
May 27, 2005
Contact name
Ludger Klein-Hitpass
E-mail(s)
ludger.klein-hitpass@uni-essen.de
Phone
+49 201 723 85552
Organization name
Institut fuer Zellbiologie
Department
Universitaetsklinikum
Lab
BioChip Lab
Street address
Virchowstr. 173
City
Essen
ZIP/Postal code
D-45122
Country
Germany
Platform ID
GPL341
Series (2)
GSE1591
INS-1 cell lines (FLP-In T-REx)
GSE6428
HNF1b, HNF4a2 and HNF6 targets
Data table header descriptions
ID_REF
VALUE
‘signal’, a measure of the abundance of the transcript
ABS_CALL
the call in an absolute analysis that indicates whether a transcript is reliably detected (Present, P) or not detected (Absent, A)
DETECTION P-VALUE
p-value that indicates the significance level of the detection call
Data table
ID_REF
VALUE
ABS_CALL
DETECTION P-VALUE
AFFX-BioB-5_at
406.4
P
0.000972
AFFX-BioB-M_at
695
P
0.00034
AFFX-BioB-3_at
239.3
P
0.004017
AFFX-BioC-5_at
806.7
P
0.00007
AFFX-BioC-3_at
479.2
P
0.000044
AFFX-BioDn-5_at
1267.3
P
0.000509
AFFX-BioDn-3_at
4756.4
P
0.000127
AFFX-CreX-5_at
8141.3
P
0.000044
AFFX-CreX-3_at
14323
P
0.000044
AFFX-DapX-5_at
141.6
P
0.001248
AFFX-DapX-M_at
420.7
P
0.006187
AFFX-DapX-3_at
347.5
P
0.00034
AFFX-LysX-5_at
1182.3
P
0.000044
AFFX-LysX-M_at
1657
P
0.000044
AFFX-LysX-3_at
2971.9
P
0.000044
AFFX-PheX-5_at
6542.9
P
0.000044
AFFX-PheX-M_at
8067.5
P
0.000044
AFFX-PheX-3_at
8702.3
P
0.000044
AFFX-ThrX-5_at
1109.1
P
0.000044
AFFX-ThrX-M_at
1462.9
P
0.000044
Total number of rows: 15923 Table truncated, full table size 431 Kbytes .Supplementary data files not provided