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Status |
Public on Jul 01, 2020 |
Title |
WT rep1 |
Sample type |
SRA |
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Source name |
Tail tendon
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Organism |
Mus musculus |
Characteristics |
tissue: tail tendon strain: C57BL/6 age: 3-week-old genotype: WT
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Extracted molecule |
total RNA |
Extraction protocol |
Tail tendons were isolated and minced, and RNA was harvested using Trizol reagent. cDNA library was prepared using the Ion Total RNA-Seq Kit v2(Life Technologies Corporation, Carlsbad, USA) according to the manufacturers protocol.
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Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Ion Torrent Proton |
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Data processing |
Clean reads were obtained from the raw reads by removing the adaptor sequences, reads with >5% ambiguous bases (noted as N) and low-quality reads containing more than 20 percent of bases with qualities of <13. The clean reads were then aligned to mouse genome (mm9) using the MapSplice program (v2.1.6) Differentially expressed genes was identified with EB-Seq algorithm Genome_build: mm9 Supplementary_files_format_and_content: tab-delimited text files include count values for each sample
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Submission date |
Aug 22, 2017 |
Last update date |
Jul 01, 2020 |
Contact name |
HONGWEI OUYANG |
E-mail(s) |
hwoy@zju.edu.cn
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Phone |
13958108337
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Organization name |
Zhejiang University
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Street address |
866 Yu Hang Tang Road,Hangzhou, Zhejiang, P.R.China.
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City |
Hangzhou |
State/province |
Zhejiang |
ZIP/Postal code |
310058 |
Country |
China |
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Platform ID |
GPL18635 |
Series (2) |
GSE102926 |
Transcriptomic Analysis of Wild Type and Mkx-/- Tendon by Next Generation Sequencing |
GSE102931 |
Alteration of transcriptomic and epigenetic landscapes in normal and heterotopic ossified tendons |
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Relations |
BioSample |
SAMN07533398 |
SRA |
SRX3111006 |
Supplementary data files not provided |
SRA Run Selector |
Raw data are available in SRA |
Processed data are available on Series record |
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