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| Status |
Public on Jul 01, 2020 |
| Title |
Mkx-ko rep1 |
| Sample type |
SRA |
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| Source name |
Tail tendon
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| Organism |
Mus musculus |
| Characteristics |
tissue: tail tendon
strain: C57BL/6
age: 3-week-old
genotype: Mkx-/-
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| Extracted molecule |
total RNA |
| Extraction protocol |
Tail tendons were isolated and minced, and RNA was harvested using Trizol reagent.
cDNA library was prepared using the Ion Total RNA-Seq Kit v2(Life Technologies Corporation, Carlsbad, USA) according to the manufacturers protocol.
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Ion Torrent Proton |
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| Data processing |
Clean reads were obtained from the raw reads by removing the adaptor sequences, reads with >5% ambiguous bases (noted as N) and low-quality reads containing more than 20 percent of bases with qualities of <13.
The clean reads were then aligned to mouse genome (mm9) using the MapSplice program (v2.1.6)
Differentially expressed genes was identified with EB-Seq algorithm
Genome_build: mm9
Supplementary_files_format_and_content: tab-delimited text files include count values for each sample
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| Submission date |
Aug 22, 2017 |
| Last update date |
Jul 01, 2020 |
| Contact name |
HONGWEI OUYANG |
| E-mail(s) |
hwoy@zju.edu.cn
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| Phone |
13958108337
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| Organization name |
Zhejiang University
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| Street address |
866 Yu Hang Tang Road,Hangzhou, Zhejiang, P.R.China.
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| City |
Hangzhou |
| State/province |
Zhejiang |
| ZIP/Postal code |
310058 |
| Country |
China |
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| Platform ID |
GPL18635 |
| Series (2) |
| GSE102926 |
Transcriptomic Analysis of Wild Type and Mkx-/- Tendon by Next Generation Sequencing |
| GSE102931 |
Alteration of transcriptomic and epigenetic landscapes in normal and heterotopic ossified tendons |
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| Relations |
| BioSample |
SAMN07533395 |
| SRA |
SRX3111009 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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