|
| Status |
Public on Aug 29, 2017 |
| Title |
Jugular-28Days-3 |
| Sample type |
RNA |
| |
|
| Source name |
Jugular-28Days
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: Wistar
tissue: Jugular vein
time: 28 days
status: arterialized
|
| Treatment protocol |
The right external jugular vein was connected to the common carotid artery by end-to-end anastomosis using a 10.0 silk suture (Ethicon Inc., Somerville, NJ).
|
| Growth protocol |
Wistar rats (250-350g) were anesthetized with a mixture of ketamine (50 mg/kg) and xylazine (10mg/kg).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Arterialized vein segments were collected at 3 days (n=3) and 28 days (n=3) after surgery. Normal jugular veins (n=5) and carotid arteries (n=2) were used as controls.
|
| Label |
Cy5
|
| Label protocol |
Biotin-labelled cRNA was produced form 1 ug of total RNA. First and second-strand cDNA was synthesized according to manufacturer’s instruction. cRNA was obtained by in vitro transcription reaction.
|
| |
|
| Hybridization protocol |
Ten microgram of fragmented cRNA was incubated with slide array for 24h at 37 ºC, under 300 RPM shaking to ensure homogenization.
|
| Scan protocol |
Slides were scanned using GenePix 4000B Array Scanner using Scan Codelink settings.
|
| Description |
Samples obtained from wistar rats
|
| Data processing |
The raw data were normalized using CyclicLoess, the most effective method for CodeLink Bioarray data. Data was also filtered for good intensity of the signal and removing genes with a low-intensity signal in at least 50% of the samples of each group.
|
| |
|
| Submission date |
Aug 28, 2017 |
| Last update date |
Jan 23, 2018 |
| Contact name |
Vinicius Bassaneze |
| Organization name |
Heart Institute
|
| Department |
Cardiopneumology
|
| Lab |
Laboratory of Genetics and Molecular Cardiology
|
| Street address |
Av. Dr. Eneas de Carvalho Aguiar, 44
|
| City |
Sao Paulo |
| State/province |
SP |
| ZIP/Postal code |
05403-900 |
| Country |
Brazil |
| |
|
| Platform ID |
GPL23957 |
| Series (1) |
| GSE103151 |
Temporal expression profiling of Arterial Remodeling in Rats |
|
