|
| Status |
Public on Nov 08, 2017 |
| Title |
AML181_shBCAT1_#1 |
| Sample type |
RNA |
| |
|
| Source name |
AML181
|
| Organism |
Homo sapiens |
| Characteristics |
treatment: KD BCAT1
|
| Treatment protocol |
Transduction with shBCAT1-3 or shNT, FACS sort of transduced cells 48hrs after the transduction
|
| Growth protocol |
AML samples were cultured in StemSpan SFEM (Stemcell Technologies) supplemented with rhIL3 (20 ng/ml), rhFLT3L (100 ng/ml), rhTPO (50 ng/ml), rhSCF (100 ng/ml, all Peprotech), 1% Penicillin/Streptomycin (Sigma) and 2 mM Glutamine (Sigma).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Rneasy (Qiagen)
|
| Label |
biotin
|
| Label protocol |
The Affymetrix 3’ IVT Pico Reagent Kit is used to generate labeled single-stranded cRNA from input amounts of 500pg total RNA
|
| |
|
| Hybridization protocol |
6,6 ug of fragmented and labeled ds-cDNA were hybridized for 17 hr at 45°C on Affymetrix GeneChip® Human Genome U133 Plus 2.0 Arrays
|
| Scan protocol |
Gene Expression Microarrays were scanned using the Affymetrix GeneChip® Scanner 3000 according to GeneChip® Expression Wash, Stain and Scan Manual for Cartridge Arrays
|
| Data processing |
The data were normalised using quantile normalisation with R using function normalize.quantiles from Bioconductor package preprocessCore
|
| |
|
| Submission date |
Sep 18, 2017 |
| Last update date |
Jan 23, 2018 |
| Contact name |
Simon Raffel |
| E-mail(s) |
s.raffel@dkfz-heidelberg.de
|
| Organization name |
German Cancer Research Center
|
| Street address |
INF 280
|
| City |
Heidelberg |
| ZIP/Postal code |
69120 |
| Country |
Germany |
| |
|
| Platform ID |
GPL570 |
| Series (2) |
| GSE100784 |
Role of Branched Chain Amino Acid Transaminase 1 (BCAT1) in Acute Myeloid Leukemia |
| GSE103960 |
Role of Branched Chain Amino Acid Transaminase 1 (BCAT1) in Acute Myeloid Leukemia [expression_BCAT1-KD #2] |
|
