|
| Status |
Public on May 01, 2009 |
| Title |
p53 +/- Whole Mouse Embryo Control A |
| Sample type |
RNA |
| |
|
| Source name |
p53 +/- Whole Mouse Embryo Control
|
| Organism |
Mus musculus |
| Characteristics |
C57BL/6, p53 +/- males and females, GD8.5-9 whole embryos collected 5h after control treatment.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
3-7 whole embryos were placed and pooled in RNAlater (Ambion), and the total RNA was extracted using mirVana™ miRNA Isolation Kit (Ambion) according to the manufacturer's instructions. The concentration and quality of the total RNA were assessed using a Nanodrop (Nanodrop) and Agilent 2100 Bioanalyzer (Agilent Technologies) respectively.
|
| Label |
biotin, Alexa-647 Streptavidin
|
| Label protocol |
CodeLink Gene Expression System: Manual Labelled cRNA Target Preparation according to manufacturer's instructions http://www.appliedmicroarrays.com/pdf/iexpress2006_web.pdf
|
| |
|
| Hybridization protocol |
CodeLink Gene Expression System: Single-Assay Bioarray Hybridization and Detection according to manufacturer's instructions http://www.appliedmicroarrays.com/pdf/codelink_bioarray_processing_protocol.pdf
|
| Scan protocol |
CodeLink Gene Expression System: Single-Assay Bioarray Hybridization and Detection according to manufacturer's instructions http://www.appliedmicroarrays.com/application_notes/63005079.pdf
|
| Description |
IACUC approval was obtained for all aspects of the animal care and treatment.
|
| Data processing |
Median normalized values derived from CodeLink Expression Analysis Version 4.1.0.29054
|
| |
|
| Submission date |
May 01, 2008 |
| Last update date |
Jul 09, 2008 |
| Contact name |
Jennifer Goldsby |
| E-mail(s) |
jsgoldsby@tamu.edu
|
| Phone |
979-845-3908
|
| Organization name |
Texas A&M University
|
| Department |
Nutrition and Food Sciences
|
| Street address |
112 Cater-Mattil
|
| City |
College Station |
| State/province |
TX |
| ZIP/Postal code |
77845 |
| Country |
USA |
| |
|
| Platform ID |
GPL2897 |
| Series (2) |
| GSE11321 |
Gene Expression Differences in p53-Deficient Day 8.5 Mouse Embryos |
| GSE11547 |
Hyperthermia and p53 effects on global gene expressions |
|
