|
| Status |
Public on Jul 24, 2018 |
| Title |
Sample_161209_PGR_D04_1140213_04 |
| Sample type |
RNA |
| |
|
| Source name |
Human Peripheral Blood
|
| Organism |
Homo sapiens |
| Characteristics |
phenotype: subclinical acute rejection
tissue: peripheral blood
|
| Extracted molecule |
total RNA |
| Extraction protocol |
PAXgene extraction of total RNA was performed using the PAXgene RNA extraction kit according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized, washed and stained in the Affymetrix Gene Titan MC
|
| Scan protocol |
GeneChips were scanned in the Affymetrix Gene Titan MC
|
| Data processing |
From the CEL files, data was generated by correction and normalization parameters using frozen RMA (fRMA). Technical batches were then adjusted using ComBat using the surrogate variable analysis (SVA) package
|
| |
|
| Submission date |
Nov 29, 2017 |
| Last update date |
Jul 25, 2018 |
| Contact name |
Sunil Kurian |
| E-mail(s) |
smkurian@scripps.edu
|
| Phone |
858-784-7759
|
| Organization name |
The Scripps Research Institute
|
| Department |
Molecular and Experimental Medicine
|
| Street address |
10550 N Torrey Pines Road
|
| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92037 |
| Country |
USA |
| |
|
| Platform ID |
GPL13158 |
| Series (2) |
| GSE107503 |
Gene expression profiling of subclinical acute kidney rejection I |
| GSE107509 |
Gene expression profiling of subclinical acute kidney rejection |
|
