|
| Status |
Public on Dec 28, 2019 |
| Title |
SAMPLE 5_HCT116 p21-/-_EMT |
| Sample type |
RNA |
| |
|
| Source name |
HCT116 p21-/-
|
| Organism |
Homo sapiens |
| Characteristics |
cell line (background): HCT116
tissue: Colorectal cancer
cell type: Human colorectal carcinoma cell line
genotype/variation: Depletion of the p21 gene
|
| Treatment protocol |
No treatment was performed
|
| Growth protocol |
HCT116 cell lines were cultured at 37°C in a humidified atmosphere with 5% CO2. Cells were harvested at a confluency of ~60-80%.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
mRNA was extracted using the QIAzol Kit from Qiagen. mRNA was purified using the Rneasy Mini Kit from Qiagen. cDNA was produced equal amounts of RNA using the RT² First Strand Kit from Qiagen.
|
| Label |
SYBR Green
|
| Label protocol |
RT-qPCR analysis was performed following the manufacturer's instructions using the Epithelial to Mesenchymal Transition RT² Profiler PCR Array from Qiagen. The CFX96TM Real-Time System and the C1000TM Thermal Cycler from Bio-Rad were used to perform the RT-PCR runs.
|
| |
|
| Hybridization protocol |
n/a
|
| Scan protocol |
n/a
|
| Description |
Test Sample 1
|
| Data processing |
The normalization and all the data analysis were performed according to the manufacturers instructions using their web-based software package: http://sabiosciences.com/pcrarraydataanalysis.php. The two different cell lines were analyzed in three biological triplicates. The mean expression Ct values of the target genes were normalized against the mean Ct value for B2M to calculate the Fold Change values.
Fold Change worksheet reports mean Ct values of normalized test samples / mean Ct values of normalized control (i.e., HCT p21-/-/HCT WT) ratios.
|
| |
|
| Submission date |
Dec 04, 2017 |
| Last update date |
Dec 28, 2019 |
| Contact name |
Pablo Lindner |
| E-mail(s) |
pablo.lindner@gmx.de
|
| Organization name |
Universityclinic Erlangen
|
| Department |
Pathology
|
| Lab |
Ag Exp. Tumorpathology
|
| Street address |
Universitaetsstr. 22
|
| City |
Erlangen |
| State/province |
Bavaria |
| ZIP/Postal code |
91054 |
| Country |
Germany |
| |
|
| Platform ID |
GPL24335 |
| Series (2) |
| GSE107665 |
RT² Profiler PCR Array analysis of different human HCT116 cell lines [Epithelial to Mesenchymal Transition] |
| GSE107666 |
RT² Profiler PCR Array analysis of different human HCT116 cell lines |
|
