|
| Status |
Public on May 10, 2008 |
| Title |
Primary central nervous system lymphoma replicate 4 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Primary central nervous system lymphoma
|
| Organism |
Homo sapiens |
| Characteristics |
Tissue: brain
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from the dissected lymphoma tissue using a kit from QIAGEN (Rneasy mini kit). The mRNA was amplified with RiboAmp HS RNA Amplification Kit
|
| Label |
Alexa Fluor 555
|
| Label protocol |
Amplified RNA (aRNA) preparations were labeled either with Alexa Fluor 555 (lymphoma sample) or Alexa Fluor 647 (reference RNA) from Invitrogen
|
| |
|
| Channel 2 |
| Source name |
Stratagene Universal human RNA
|
| Organism |
Homo sapiens |
| Characteristics |
reference
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from the dissected lymphoma tissue using a kit from QIAGEN (Rneasy mini kit). The mRNA was amplified with RiboAmp HS RNA Amplification Kit
|
| Label |
Alexa Fluor 647
|
| Label protocol |
Amplified RNA (aRNA) preparations were labeled either with Alexa Fluor 555 (lymphoma sample) or Alexa Fluor 647 (reference RNA) from Invitrogen
|
| |
|
| |
| Hybridization protocol |
The labeled samples were hybridized in Agilent microarray chambers (G2534A) at 60°C for 17 hours
|
| Scan protocol |
Images were scanned using Confocal laser scanner and quantified using Imagene software (version 6)
|
| Description |
Fresh frozen samples from immunocompetent patients
|
| Data processing |
Data was background subtracted and lowess normalized using GeneSpring (version 7.2) software
|
| |
|
| Submission date |
May 09, 2008 |
| Last update date |
Jul 19, 2013 |
| Contact name |
Peter J Park |
| E-mail(s) |
peter_park@harvard.edu
|
| Phone |
617-432-7373
|
| Organization name |
Harvard Medical School
|
| Department |
Center for Biomedical Informatics
|
| Street address |
10 Shattuck St
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platform ID |
GPL6848 |
| Series (1) |
| GSE11392 |
Pathway analysis of primary central nervous system lymphoma (PCNSL) |
|
