|
Status |
Public on Dec 28, 2008 |
Title |
PrEn_d3 #1 (251508710460 Array1_3) |
Sample type |
RNA |
|
|
Channel 1 |
Source name |
PrEn_d3
|
Organism |
Mus musculus |
Characteristics |
embryonic carcinomal cell Strain: 129 Gender: unknown sex Age: TBE Individual Identifier: PE4 RA-A day3 251508710460 Array1_3
|
Biomaterial provider |
Kazuhiro Aiba,Laboratory of Genetics, NIA/NIH. aibaka@mail.nih.gov, kom@mail.nih.gov
|
Treatment protocol |
grown on gelatin in DMEM (GIBCO 11965-092) with 10%FCS, 2mM Glutamine and Penicillin-Streptomycin. 3 days treatment with 100nM RA and 1mM dibutyryl cAMP.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA extracted with TRIzol reagent (Invitrogen) according to the manufacturers instructions.
|
Label |
Cy3
|
Label protocol |
Total RNA was labeled using Agilents Fluorescent Linear Amplification kit, according to manufacturers instructions (Product Number G2554A or P/N G2556-66002, Version 3.0, June 2002).
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|
|
Channel 2 |
Source name |
Universal Mouse Reference 129ES Cell
|
Organism |
Mus musculus |
Characteristics |
Universale Mouse Reference
|
Biomaterial provider |
Stratagene
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted from cultured cells using a Stratagene Absolutely RNA kit. DNase-treated total RNA samples from 11 cultured cell lines derived from embryo, embryo fibroblast, kidney, liver/hepatocyte, lung/alveolar macrophage, B-lymphocyte, T-lymphocyte (thymus), mammary gland, muscle myoblast, skin, and testis were pooled in equal parts. The resulting URM RNA was mixed with 129ES cell RNA (Lif+) at 2:1 ratio and 2.5ug of mixed RNA was used for labeling in each tube
|
Label |
Cy5
|
Label protocol |
Total RNA was labeled using Agilents Low RNA Input Fluorescent Linear Amplification kit, according to manufacturers instructions.
|
|
|
|
Hybridization protocol |
Slides were hybridized according to manufacturers protocol (Two-Color Microarray-Based Genr Expression Analysis Protocol, Product # G4140-90050, Version 5.0.1, August 2006)
|
Scan protocol |
Slides were scanned with an Agilent DNA Microarray Scanner (model G2505-64120) at 100% and 10% PMT in both channels, with a scan resolution of 5um.
|
Description |
PrEn_d3
|
Data processing |
Data are extracted with Agilent Feature Extraction Software.The data were further processed with NIA ANOVA tool utilities.See http://lgsun.grc.nia.nih.gov/ANOVA for details.
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|
|
Submission date |
May 21, 2008 |
Last update date |
Dec 28, 2008 |
Contact name |
Minoru S.H. Ko |
E-mail(s) |
kom@mail.nih.gov
|
Phone |
410-558-8359
|
Organization name |
NIH
|
Department |
National Institute on Aging
|
Lab |
Lab of Genetics
|
Street address |
251 Bayview Blvd, Suite 100, 10C
|
City |
Baltimore |
State/province |
MD |
ZIP/Postal code |
21224 |
Country |
USA |
|
|
Platform ID |
GPL6867 |
Series (1) |
GSE11523 |
Defining Developmental Potency and Cell Lineage Trajectories by Expression Profiling of Differentiating Mouse ES Cells |
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