|
| Status |
Public on Feb 23, 2018 |
| Title |
WD015_FASD |
| Sample type |
genomic |
| |
|
| Source name |
WD015
|
| Organism |
Homo sapiens |
| Characteristics |
individual: WD015
cell type: Buccal epithelial cells
Sex: M
disease status: FASD
disease subgroup: pFAS
age: 9
self-reported ethnicity: First Nations
sentrix id: 101070190067
sentrix position: R01C02
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was isolated using the Isohelix DNA isolation kit and bisulfite converted with the Zymo EZ DNA Methylation Kit, as per manufacturer’s instructions.
|
| Label |
Cy5 and Cy3
|
| Label protocol |
Standard Illumina Protocol
|
| |
|
| Hybridization protocol |
Bisulfite converted DNA was amplified, fragmented and hybridized to Illumina Infinium HumanMethylation450k Beadchip using standard Illumina protocol
|
| Scan protocol |
Arrays were imaged with BeadArray Reader using standard recommended Illumina scanner setting
|
| Description |
WD015_1
|
| Data processing |
GenomeStudio software V2011.1 with GenomeStudio Methylation Analysis Module Version 1.9.1000 (background-subtraction, colour-correction)
|
| |
|
| Submission date |
Jan 14, 2018 |
| Last update date |
Feb 23, 2018 |
| Contact name |
Michael S. Kobor |
| Organization name |
Centre for Molecular Medicine and Therapeutics / University of British Columbia
|
| Department |
Medical Genetics
|
| Lab |
Kobor
|
| Street address |
950 West 28th Avenue
|
| City |
Vancouver |
| State/province |
BC |
| ZIP/Postal code |
V5Z 4H4 |
| Country |
Canada |
| |
|
| Platform ID |
GPL13534 |
| Series (1) |
| GSE109042 |
DNA methylation as a predictor of fetal alcohol spectrum disorder |
|
