|
| Status |
Public on Feb 06, 2018 |
| Title |
5500024051907020909371_C04 |
| Sample type |
RNA |
| |
|
| Source name |
Whole blood
|
| Organism |
Homo sapiens |
| Characteristics |
time: 0
pid_dummy: P8
dose: 5
trial: IM124001
sample_isolation: Male NHVs were randomly assigned to receive daily doses of 5, 10, or 30 mg prednisolone for 9 days. Two participants received placebo. PAXgene tubes were collected before dosing and at 2, 4, 8, 48, 144, and 216 hours postdose.
|
| Treatment protocol |
PAXgene tubes were stored at -20C until processing for RNA extraction.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated, treated with DNase I, and cleaned up using a Qiagen RNeasy MinElute Cleanup Kit. RNA concentrations were determined using NanoDrop (Thermo Fisher Scientific, Waltham, MA, USA) and RNA quality was evaluated using the Experion electrophoresis system (Bio-Rad Laboratories, Hercules, CA, USA).
|
| Label |
biotin
|
| Label protocol |
Double-stranded cDNAs were synthesized from 1 μg of total RNA through reverse transcription with an oligo-dT primer containing the T7 RNA polymerase promoter and converted to double-strand using the cDNA Synthesis System (Invitrogen). Biotin-labeled cRNA was generated from the cDNA. All cDNA and cRNA target preparation steps were processed on a Caliper GeneChip Array Station from Affymetrix.
|
| |
|
| Hybridization protocol |
Biotin-labeled cRNA was generated from the cDNA and used to probe a Human Genome HT_HG-U133A plate (Affymetrix, Sunnyvale, CA, USA) Array hybridization, washing, and scanning were performed according to the manufacturer’s recommendations.
|
| Scan protocol |
Array hybridization, washing, and scanning were performed according to the manufacturer’s recommendations.
|
| Data processing |
CEL files from the Affymetrix Array Station were processed and normalized by the Robust Multi-Array Average (RMA) algorithm
|
| |
|
| Submission date |
Feb 05, 2018 |
| Last update date |
Feb 06, 2018 |
| Contact name |
Hanjoong Jo |
| E-mail(s) |
hanjoong.jo@bme.gatech.edu
|
| Phone |
(404) 712-9654
|
| Organization name |
Georgia Tech and Emory Univ
|
| Department |
Coulter Department of Biomedical Engineering
|
| Street address |
1760 Haygood Drive, Health Sciences Research Bldg E170
|
| City |
Atlanta |
| State/province |
GA |
| ZIP/Postal code |
30322 |
| Country |
USA |
| |
|
| Platform ID |
GPL3921 |
| Series (1) |
|
