|
| Status |
Public on Jun 15, 2009 |
| Title |
control, day 160 of pregnancy, 1st replicate |
| Sample type |
RNA |
| |
|
| Source name |
Total white blood cells from ncpBVDV-infected heifers with PI, TI or control fetuses
|
| Organism |
Bos taurus |
| Characteristics |
Total white blood cells
|
| Treatment protocol |
Blood sample collected at day 160 of gestation
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Extraction of total RNA was performed from Trireagent BD for blood derivatives according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard one-cycle Affymetrix protocol from 3.5 micrograms total RNA (Expression Analysis Technical Manual, 2004, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 micrograms of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse GeneChip 430A 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GCOS Scanner 7G.
|
| Description |
Gene expression after ncpBVDV infection
|
| Data processing |
Data was analyzed with DChip 2006, build 12.21.06, using default settings for model-based summation of expression values, and following normalization to invariant set of probes.
|
| |
|
| Submission date |
Jun 19, 2008 |
| Last update date |
Jun 23, 2008 |
| Contact name |
Kate McInnerney |
| E-mail(s) |
kmcinnerney@montana.edu
|
| Phone |
406-994-5666
|
| Fax |
406-994-4926
|
| URL |
http://cores.montana.edu
|
| Organization name |
Montana State University
|
| Department |
Microbiology
|
| Lab |
Functional Genomics Facility
|
| Street address |
109 Lewis Hall
|
| City |
Bozeman |
| State/province |
MT |
| ZIP/Postal code |
59717 |
| Country |
USA |
| |
|
| Platform ID |
GPL2112 |
| Series (1) |
| GSE11835 |
Gene expression data in heifers with persistently infected (PI) or transiently infected (TI) fetuses. |
|
