|
| Status |
Public on Aug 08, 2019 |
| Title |
4H_4OHT_rep3 (ActoD) |
| Sample type |
RNA |
| |
|
| Source name |
mouse ESCs
|
| Organism |
Mus musculus |
| Characteristics |
cell type: ESCs
treatment: Actinomycin D for 30 mins then a subsequent 4 hours supplemented with 4OHT
|
| Treatment protocol |
cells left untreated, or treated with 5μM Actinomycin D. 30 minutes later they were treated with or without 250nM 4OHT for 4 hours
|
| Growth protocol |
Cells were grown in standard ESC medium supplemented with LIF and 250nM PD173074
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was collected using the RNeasy Mini Kit (Qiagen). Genomic DNA was eliminated by DNAse treatment (Qiagen).
|
| Label |
Cy5
|
| Label protocol |
RNA was amplified into cRNA and 100ng of total RNA was labeled using the Low Input Quick Amp Labeling Kit (Agilent Technologies- 5190-2305).
|
| |
|
| Hybridization protocol |
Samples were then hybridized to the Agilent-028005 SurePrint G3 Mouse GE 8x60K Microarray (60000 probes, Agilent Technologies).
|
| Scan protocol |
Images were scanned using the high sensitivity protocol (AgilentG3_HiSen_GX_1color) on a SureScan microarray scanner (Agilent Technologies) and probe intensities were obtained by taking the gProcessedSignal from the output of Agilent feature extraction software using default settings.
|
| Description |
Gene expression data from cells treated with Actinomycin D for 30 mins then a subsequent 4 hours supplemented with 4OHT
|
| Data processing |
Probe intensities were analysed using the NIA Array Analysis tool (http://lgsun.grc.nia.nih.gov/ANOVA/).
|
| |
|
| Submission date |
Feb 15, 2018 |
| Last update date |
Aug 08, 2019 |
| Contact name |
Josh M Brickman |
| E-mail(s) |
joshua.brickman@sund.ku.dk
|
| Organization name |
Copenhagen University
|
| Department |
reNEW
|
| Lab |
Brickman
|
| Street address |
3B Blegdamsvej
|
| City |
Copenhagen |
| ZIP/Postal code |
2200 |
| Country |
Denmark |
| |
|
| Platform ID |
GPL13912 |
| Series (2) |
| GSE110664 |
Erk regulated mRNA degradation |
| GSE132444 |
Dynamic Lineage Priming by ERK is Driven by Transcription Factor-Independent Enhancer Regulation |
|
