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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Apr 25, 2018 |
| Title |
4t1-control |
| Sample type |
SRA |
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| Source name |
breast cancer cell
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| Organism |
Mus musculus |
| Characteristics |
treatment: control-treated 4T1 group
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| Extracted molecule |
total RNA |
| Extraction protocol |
RNA extracted using Trizol by following manufacturer’s instruction (Invitrogen).
RNA libraries were constructed by using rRNA-depleted RNAs with TruSeq Stranded Total RNA Library Prep Kit (Illumina, San Diego, CA, USA) according to the manufacturer’s instructions. Libraries were controlled for quality and quantified using the BioAnalyzer 2100 system (Agilent Technologies, Inc., USA).
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
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| Data processing |
Basecalls performed using CASAVA version 1.8
RNA seq reads were adaptor-trimmed and mapped to mouse genome(mm9) by bowtie2 software with default parameters.
Genome_build: mm9
Supplementary_files_format_and_content: normalized counts
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| Submission date |
Feb 18, 2018 |
| Last update date |
Apr 25, 2018 |
| Contact name |
Shengtao Zhou |
| E-mail(s) |
taotaovip2005@163.com
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| Organization name |
West China Second Hospital, Sichuan University
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| Department |
Department of Obstetrics and Gynecology
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| Street address |
#17, Renmin South Road
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| City |
Chengdu |
| ZIP/Postal code |
610041 |
| Country |
China |
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| Platform ID |
GPL13112 |
| Series (1) |
| GSE110770 |
Profiling of differential RNAs in LY500307-treated 4T1 cells and control cells |
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| Relations |
| BioSample |
SAMN08563503 |
| SRA |
SRX3720537 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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