|
| Status |
Public on Dec 31, 2008 |
| Title |
Doxo-treated heart, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
rat heart
|
| Organism |
Rattus norvegicus |
| Characteristics |
Wistar
isolated heart, Langendorff-perfused
|
| Treatment protocol |
Hearts from male Wistar rats (ca. 300 g) were perfused in a noncirculating Langendorff apparatus without (control; CO) or with 2 M doxorubicin (DO) during 2 hours.
|
| Growth protocol |
n.a.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using RNAeasy Mini kit (Qiagen) according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip® Rat Genome 230 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
|
| Description |
Gene expression data from rat hearts perfused for 2 hours with addition of 2uM doxorubicin
|
| Data processing |
Normalization and computation of expression values was performed using the Robust Multichip Average (RMA) algorithm.
|
| |
|
| Submission date |
Jul 28, 2008 |
| Last update date |
Jul 28, 2008 |
| Contact name |
Michael Zaugg |
| E-mail(s) |
michael.zaugg@usz.ch
|
| Phone |
+41-1-255 11 11
|
| Organization name |
Anesthesiology, University Hospital Zurich
|
| Department |
Anesthesiology
|
| Lab |
Perioperative Cardioprotection Research Group
|
| Street address |
Raemistrasse 100
|
| City |
Zurich |
| ZIP/Postal code |
8091 |
| Country |
Switzerland |
| |
|
| Platform ID |
GPL1355 |
| Series (1) |
| GSE12260 |
Expression data from isolated perfused rat hearts exposed to doxorubicin |
|
