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Sample GSM3090346 Query DataSets for GSM3090346
Status Public on Apr 10, 2018
Title B.IFNg.2hr.Sp#2
Sample type RNA
 
Source name B.IFNg.2hr.Sp
Organism Mus musculus
Characteristics genetic background: B6
Sex: Male
age: 6 Weeks Old
issue: Spleen
exclusion markers: TCRB-
phenotype markers: CD19+ IgM+
separation method: FACSAria
Extracted molecule total RNA
Extraction protocol TriZol
Label Biotin
Label protocol Affymetrix GeneChip WT Terminal Labeling and Controls Kit
 
Hybridization protocol Ambion WT Expression Kit
Scan protocol Affymetrix GeneChip Scanner
Data processing The datasets was pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
 
Submission date Apr 09, 2018
Last update date Apr 10, 2018
Contact name CBDM Lab
E-mail(s) cbdm@hms.harvard.edu
Phone 617-432-7747
Organization name Harvard Medical School
Department Microbiology and Immunobiology
Lab CBDM
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02215
Country USA
 
Platform ID GPL6246
Series (2)
GSE75306 ImmGen Cytokines: Interferons
GSE112876 Cell specific response to IFNg

Data table header descriptions
ID_REF
VALUE RMA normalized signal intensity

Data table
ID_REF VALUE
10344620 31.7123
10344622 267.2871
10344624 899.0252
10344633 1205.8033
10344637 409.15
10344653 38.5439
10344658 427.7889
10344674 20.3415
10344679 49.4599
10344707 644.9016
10344713 608.831
10344723 109.5503
10344725 87.8056
10344741 952.3768
10344743 44.8419
10344750 620.6633
10344772 52.8461
10344789 436.2244
10344797 548.4229
10344799 532.5694

Total number of rows: 24922

Table truncated, full table size 428 Kbytes.




Supplementary file Size Download File type/resource
GSM3090346_EA07068_374454_MOGENE-1_0-ST-V1_B.IFNG.2HR.SP_2.CEL.gz 4.3 Mb (ftp)(http) CEL
Processed data included within Sample table

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