genetic background: B6 Sex: Male age: 6 Weeks Old issue: Peritoneal Cavity phenotype markers: CD19+ CD43+ CD5+ separation method: FACSAria
Extracted molecule
total RNA
Extraction protocol
TriZol
Label
Biotin
Label protocol
Affymetrix GeneChip WT Terminal Labeling and Controls Kit
Hybridization protocol
Ambion WT Expression Kit
Scan protocol
Affymetrix GeneChip Scanner
Data processing
The datasets was pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
