|
| Status |
Public on Aug 01, 2010 |
| Title |
Left ventricles shunt rep2 (set 1) |
| Sample type |
RNA |
| |
|
| Source name |
Left ventricular tissue following 28 days aorto-caval shunt operation
|
| Organism |
Rattus norvegicus |
| Characteristics |
Strain: Sprague-Dawley; Gender: male; Weight: 225-275 g; Tissue: Heart atria and ventricles
|
| Treatment protocol |
Tissue was obtained from Sprague Dawley rats, sacrificed by decapitation. The tissue was snap-frozen in liquid nitrogen and stored at -80 ºC until RNA extraction
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Qiagen mini columns according to the manufacturer's instructions.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug of total RNA (Expression Analysis Technical Manual, 2004, Affymetrix).
|
| |
|
| Hybridization protocol |
A total of 20 ug of fragmented cRNA was hybridized to each GeneChip® Rat Genome 230 2.0 array. After 16 hours of incubation at 45 C, the GeneChips® were washed and stained with Streptavidin- phycoerythrin biotinylated anti-streptavidin antibody in the Affymetrix GeneChip® Fluidics Station 400
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip® 3000 Scanner
|
| Description |
n/a
|
| Data processing |
The data was analyzed with Robust Multiple-Array Averaging (RMA) which uses a model based background correction, quantile normalization and robust averaging expression summary method.
|
| |
|
| Submission date |
Sep 12, 2008 |
| Last update date |
Aug 24, 2009 |
| Contact name |
Adolfo J de Bold |
| E-mail(s) |
adebold@ottawaheart.ca
|
| Phone |
(613) 761-4265
|
| Fax |
(613) 761-1597
|
| Organization name |
University of Ottawa Heart Institute
|
| Department |
Cardiovascular Endocrinology
|
| Street address |
40 Ruskin St.
|
| City |
Ottawa |
| State/province |
ON |
| ZIP/Postal code |
K1Y 4W7 |
| Country |
Canada |
| |
|
| Platform ID |
GPL1355 |
| Series (2) |
| GSE12754 |
Expression data from 28 day sham and shunt atrial and ventricular tissues (set 1) |
| GSE12758 |
Expression data from 28 day sham and shunt atrial and ventricular tissues |
|
