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Status |
Public on Aug 01, 2010 |
Title |
Left ventricles shunt rep3 (set 1) |
Sample type |
RNA |
|
|
Source name |
Left ventricular tissue following 28 days aorto-caval shunt operation
|
Organism |
Rattus norvegicus |
Characteristics |
Strain: Sprague-Dawley; Gender: male; Weight: 225-275 g; Tissue: Heart atria and ventricles
|
Treatment protocol |
Tissue was obtained from Sprague Dawley rats, sacrificed by decapitation. The tissue was snap-frozen in liquid nitrogen and stored at -80 ºC until RNA extraction
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted using Qiagen mini columns according to the manufacturer's instructions.
|
Label |
Biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug of total RNA (Expression Analysis Technical Manual, 2004, Affymetrix).
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|
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Hybridization protocol |
A total of 20 ug of fragmented cRNA was hybridized to each GeneChip® Rat Genome 230 2.0 array. After 16 hours of incubation at 45 C, the GeneChips® were washed and stained with Streptavidin- phycoerythrin biotinylated anti-streptavidin antibody in the Affymetrix GeneChip® Fluidics Station 400
|
Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip® 3000 Scanner
|
Description |
n/a
|
Data processing |
The data was analyzed with Robust Multiple-Array Averaging (RMA) which uses a model based background correction, quantile normalization and robust averaging expression summary method.
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|
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Submission date |
Sep 12, 2008 |
Last update date |
Aug 24, 2009 |
Contact name |
Adolfo J de Bold |
E-mail(s) |
adebold@ottawaheart.ca
|
Phone |
(613) 761-4265
|
Fax |
(613) 761-1597
|
Organization name |
University of Ottawa Heart Institute
|
Department |
Cardiovascular Endocrinology
|
Street address |
40 Ruskin St.
|
City |
Ottawa |
State/province |
ON |
ZIP/Postal code |
K1Y 4W7 |
Country |
Canada |
|
|
Platform ID |
GPL1355 |
Series (2) |
GSE12754 |
Expression data from 28 day sham and shunt atrial and ventricular tissues (set 1) |
GSE12758 |
Expression data from 28 day sham and shunt atrial and ventricular tissues |
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