|
| Status |
Public on Nov 09, 2020 |
| Title |
P7_Ctx__Cont.rep2 |
| Sample type |
SRA |
| |
|
| Source name |
P7 mouse cerebral cortex
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
tissue: cerebral cortex of brain
age: postnatal day 7
genotype: wild type
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
Total RNA from cerebral cortical regions of brains or neuronal stem cells were extracted using Rneasy Plus Midi kit (Qiagen) according to the manufacturer’s instructions.
Libraries were prepared according to Life Techonology's instructions accompanying the Ion AB Library Builder™ System and Library Builder. Briefly, total RNA was treated with Dynabeads® mRNA DIRECT™ Microkit to prepare PolyA-selected messenger RNA. RNA libraries were then prepared using the Life Techonology Ion Total RNA-Seq Kit for the AB Library Builder™ System and Library Builder. High-throughput sequencing was performed using the Ion Proton System. Sfpq mRNA level was down-regulated less than 10% in Neuro-2a treated with Sfpq-siRNA comparing those of Luc-siRNA.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Ion Torrent Proton |
| |
|
| Data processing |
Basecalls performed using Torrent Suite version 4.0.2
The seuqence data was mapped to the genome sequence with STAR ver. 2.4.1d
Genome_build: mm10
Supplementary_files_format_and_content: Expression values for refseq gene models (GRCm38.p4) were calcurated with RSEM (ver. 1.3.0).
|
| |
|
| Submission date |
Jul 12, 2018 |
| Last update date |
Nov 09, 2020 |
| Contact name |
Kei IIDA |
| E-mail(s) |
kiida@life.kindai.ac.jp
|
| Organization name |
Kindai University
|
| Street address |
Kowakae 3-4-1
|
| City |
Higashi-Osaka |
| State/province |
Osaka |
| ZIP/Postal code |
5778502 |
| Country |
Japan |
| |
|
| Platform ID |
GPL18635 |
| Series (2) |
| GSE117016 |
RNA-binding protein quaking (Qk) promotes switching from neurogenesis to gliogenesis of neural stem cells during brain development [P7 cortex] |
| GSE117018 |
RNA-binding protein quaking (Qk) promotes switching from neurogenesis to gliogenesis of neural stem cells during brain development |
|
| Relations |
| BioSample |
SAMN09651194 |
| SRA |
SRX4385801 |
