|
| Status |
Public on Jul 20, 2018 |
| Title |
RWPE1 bi-96 PD M4 |
| Sample type |
RNA |
| |
|
| Source name |
non-malignant prostate cell line transfected with biotin-miRNA
|
| Organism |
Homo sapiens |
| Characteristics |
gender: male
cell line: RWPE-1
cell type: non-malignant prostate epithelial cell
biotin-mirna: bi-96
rna source: Pulldown
|
| Treatment protocol |
Exponentially growing cells were transfected with 30nM biotin-miR-96 or non-targeting control (bi-NC) for 24 hours.
|
| Growth protocol |
Cells maintained in Keratinocyte Serum Free Medium supplemented with EGF and Bovine Pituitary Extract.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with TRIzol reagent
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Data processing |
The data were normalised using quantile normalisation with IlluminaGUI in R
|
| |
|
| Submission date |
Jul 13, 2018 |
| Last update date |
Jul 20, 2018 |
| Contact name |
Mark D Long |
| E-mail(s) |
mark.long@roswellpark.org
|
| Organization name |
Roswell Park Comprehensive Cancer Center
|
| Department |
Bioinformatics & Biostatistics
|
| Street address |
Elm & Carlton Streets
|
| City |
Buffalo |
| State/province |
NY |
| ZIP/Postal code |
14263 |
| Country |
USA |
| |
|
| Platform ID |
GPL10558 |
| Series (2) |
| GSE117103 |
The miR-96 and RARG signaling axis governs androgen signaling and prostate cancer progression III |
| GSE117182 |
The miR-96 and RARG signaling axis governs androgen signaling and prostate cancer progression |
|
