|
| Status |
Public on Aug 01, 2018 |
| Title |
eFHLC, day 13 biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Cultured cells
|
| Organism |
Homo sapiens |
| Characteristics |
cells: Differentiated HESC
time point: day 13
|
| Treatment protocol |
No treatment
|
| Growth protocol |
Cells were grown in condition medium from fetal human hepatocytes
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HG-U133 Plus 2 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Affy Gene Chip Scanner 3000 7G.
|
| Description |
Gene expression data from early stage fetal hepatocyte-like cells
|
| Data processing |
The probe level intensities were adjusted by quantile normalization and gene expression measures were summarized by the robust multi-array analysis (RMA) approach with Bioconductor software
|
| |
|
| Submission date |
Jul 31, 2018 |
| Last update date |
Aug 01, 2018 |
| Contact name |
Sanjeev Gupta |
| E-mail(s) |
sanjeev.gupta@einsteinmed.org
|
| Organization name |
Albert Einstein College of Medicine
|
| Department |
Medicine
|
| Street address |
1300 Morris Park Ave.
|
| City |
Bronx |
| State/province |
NY |
| ZIP/Postal code |
10461 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE115410 |
Gene expression profiling of adult human hepatocytes |
|
