|
| Status |
Public on Mar 30, 2009 |
| Title |
Rat iPS F65-2 vs mix1 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Rat iPS F65-2
|
| Organism |
Rattus norvegicus |
| Characteristics |
SD Rat iPS F65 cells were maintained on irradiated primary murine embryonic feeders (PMEF).
|
| Treatment protocol |
untreated
|
| Growth protocol |
Rat iPS cells were maintained on irradiated mouse embryonic fibroblasts (MEF) in Knockout DMEM supplemented with 10% ES cell qualified fetal bovine serum, 10% KnockOut serum replacer, 0.1 mM non-essential amino acids, 1 mM L-glutamine, and 0.1 mM ß-mercaptoethanol (ES media) (all from Invitrogen, Carlsbad, CA).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA are isolated from cell pellets using TriZol reagent(Invitrogen) followed by Rneasy mini kit (Qiagen) clean-up.
|
| Label |
Cy3
|
| Label protocol |
Please see manufacturer's web site at http://www.agilent.com/
|
| |
|
| Channel 2 |
| Source name |
mix1
|
| Organism |
Rattus norvegicus |
| Characteristics |
mix1 was the mixture of Rat bone marrow cells (BMC) and Rat primary ear fibroblasts (PEF).
|
| Treatment protocol |
untreated
|
| Growth protocol |
Rat bone marrow cells (BMC) were cultured in αDMEM culture medium (Invitrogen) supplemented with 10% heat-inactivated fetal bovine serum. Rat primary ear fibroblasts (PEF) were cultured in DMEM culture medium (Invitrogen) supplemented with 10% fetal bovine serum.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA are isolated from cell pellets using TriZol reagent(Invitrogen) followed by Rneasy mini kit (Qiagen) clean-up.
|
| Label |
Cy5
|
| Label protocol |
Please see manufacturer's web site at http://www.agilent.com/
|
| |
|
| |
| Hybridization protocol |
Please see manufacturer's web site at http://www.agilent.com/
|
| Scan protocol |
Please see manufacturer's web site at http://www.agilent.com/
|
| Description |
This is one of the three repeated sample data.
|
| Data processing |
The microarray was scanned by Agilent G2565BA scanner,raw data were extracted by Feature Extraction Software. Signal intensity were normalized using Lowess method ,GeneSpring 10.0 . VALUE:Cy3/Cy5 signal intensity, ratio:Log2 transformed
|
| |
|
| Submission date |
Oct 27, 2008 |
| Last update date |
Mar 30, 2009 |
| Contact name |
Jing Liao |
| E-mail(s) |
jliao@sibs.ac.cn
|
| Phone |
86-21-54921387
|
| Fax |
86-21-54921388
|
| Organization name |
Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Science
|
| Department |
Institute of Biochemistry and Cell Biology
|
| Lab |
LeiXiao's Lab
|
| Street address |
320 Yue Yang Road
|
| City |
Shanghai |
| ZIP/Postal code |
200031 |
| Country |
China |
| |
|
| Platform ID |
GPL7294 |
| Series (2) |
| GSE13359 |
Rat iPS F65 cells' gene expression difference compared with that of the adult cells including rat BMC and PEF cells |
| GSE13462 |
Comparison of human ES cells, mouse ES cells, rat iPS cells and their somatic cells. |
|
