|
| Status |
Public on Dec 31, 2008 |
| Title |
WT_Thymus_CD4-1D |
| Sample type |
RNA |
| |
|
| Source name |
WT_Thymus_CD4
|
| Organism |
Mus musculus |
| Characteristics |
C57BL/6, Female, 8-12weeks,
Thymus, excised, 60min
|
| Treatment protocol |
anti-CD4, anti-CD8, and anti-CD44 staining
|
| Growth protocol |
RPMI+10 FCS
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy plus Mini kit (Qiagen)
|
| Label |
biotin
|
| Label protocol |
Affymetrix Mouse Exon Array labeling system
|
| |
|
| Hybridization protocol |
Affymetrix Gene Chip Fluidics station 450
|
| Scan protocol |
Affymetrix GCS3000 7G
|
| Description |
gene-level analysis; MoEx-1_0-st-v1.r2.dt1.mm8.core.mps
|
| Data processing |
Exon- and gene-level data were generated using Partek Genomic Suite 6.3 software, with the defult algorithm of RMA.
|
| |
|
| Submission date |
Oct 31, 2008 |
| Last update date |
Jan 04, 2012 |
| Contact name |
Zuopeng Wu |
| E-mail(s) |
zuopeng.wu@anu.edu.au
|
| Phone |
+61-2-61251392
|
| Organization name |
John Curtin School of Medical Research
|
| Department |
Dept. of Immunology & Genetics
|
| Lab |
Immunogenomics Lab
|
| Street address |
Garren Rd
|
| City |
Canberra |
| State/province |
ACT |
| ZIP/Postal code |
0200 |
| Country |
Australia |
| |
|
| Platform ID |
GPL6096 |
| Series (1) |
| GSE13416 |
Memory T cell RNA rearrangement by hn RNP LL |
|
| Relations |
| Alternative to |
GSM338448 (exon-level analysis) |
