|
| Status |
Public on Oct 17, 2018 |
| Title |
icKO naive MG |
| Sample type |
RNA |
| |
|
| Source name |
microglia, Cx47 deficient mice CNS
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57Black/6J
tissue: microglia
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from cells using TRIzol Reagent (nitrogen) and purified using SV Total RNA Isolation System (Promega) according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
The cRNA was amplified, labeled using GeneChip WT Pico Reagent Kit according to the manufacturer's instructions.
|
| |
|
| Hybridization protocol |
cRNA was hybridized to Affymetrix Clariom D Assay Mouse array, washed and stained according to the manufacturer's instructions.
|
| Scan protocol |
The hybridized microarray slides were scanned using an Affymetrix scanner. The relative hybridization intensities and background hybridization values were calculated using Feature Extraction Software version 9.5.1.1 (Agilent Technologies).
|
| Data processing |
The data were analyzed with median polish summarization and quantile normalization by Affymetrix® Expression Console™ 1.1 software, and obtained normalized intensity values.
|
| |
|
| Submission date |
Oct 16, 2018 |
| Last update date |
Oct 17, 2018 |
| Contact name |
Ryo Yamasaki |
| E-mail(s) |
yamasaki.ryo.510@m.kyushu-u.ac.jp
|
| Phone |
+81926425340
|
| Organization name |
Kyushu University
|
| Department |
Neurology
|
| Street address |
3-1-1 Maidashi, Higashi-Ku
|
| City |
Fukuoka |
| State/province |
Fukuoka |
| ZIP/Postal code |
812-8582 |
| Country |
Japan |
| |
|
| Platform ID |
GPL20258 |
| Series (2) |
| GSE121337 |
A novel animal model for secondary progressive multiple sclerosis by oligodendroglia-specific conditional connexin 47 knockout [Affy] |
| GSE121339 |
A novel animal model for secondary progressive multiple sclerosis by oligodendroglia-specific conditional connexin 47 knockout |
|
