Sex: male mouseid: 13 tissue: m. extensor digitorum longus (EDL) combined treatment group: Control tumor bearing (yes/no): no whole-body vibration training (wbv) (yes/no): no body mass at day 19 (as % of mean of day 1 and day 0): 108.0 carcass mass at day 19 (g): 24.9 mass muscle sample (mg): 7.9
Treatment protocol
Murine C26 adenocarcinoma cells were cultured and suspended as described previously [PMID: 19259092]. Tumour cells (1x106 cells in 0.2 ml of Hank's balanced salt solution (HBSS)) were inoculated subcutaneously into the right inguinal flank of the mice under general anaesthesia (isoflurane/N2O/O2). HBSS was used as sham injection (0.2 ml). Starting on D1 mice were subjected to a whole-body vibration training (WBV) protocol with 15 min of vibration training for 7 days/week, with a frequency of 45 Hz and 1.0 g acceleration. The vibration platform used was an adjusted commercially available VG Professional power plate (VibroGym, Badhoevedorp, The Netherlands).
Growth protocol
Twenty-four male CD2F1 mice weighing approximately 20g (BALB/c x DBA/2, Charles River, The Netherlands) were individually housed in macrolon type 3 cages with sawdust and tissues as cage enrichment, in a climate-controlled room (21 °C ± 1 °C) with a 12:12 hour dark-light cycle. Mice had ad libitum access to chow and water. Upon arrival, mice were stratified on body weight and divided into groups of 6 animals (Control [C], Control + WBV [C+V], Tumour bearing [T] and Tumour bearing + WBV [T+V]). Subsequently, mice were allowed to acclimatize for 1 week prior to the start of the experiment.
Extracted molecule
total RNA
Extraction protocol
RNA from EDL, SOL and heart was isolated (RNeasy Micro kit, Qiagen, Venlo, the Netherlands). Subsequently, RNA was quantified (Nanodrop ND1000, Nanodrop technologies Wilmington, DE, USA) and integrity was checked by an Agilent 2100 Bioanalyser with RNA 6000 microchips (Agilent Technologies, South Queensferry, UK).
Label
biotin
Label protocol
Purified total RNA (100ng per sample) was labeled with the Whole-Transcript Sense Target Assay (Affymetrix, Santa Clara, CA, USA; P/N 900652).
Hybridization protocol
Hybridization and washing of the Affymetrix GeneChip Mouse Gene 1.1 ST peg arrays were performed on a GeneTitan Instrument (Affymetrix, Santa Clara, CA) according to the manufacturer's recommendations.
Scan protocol
Arrays were scanned on an Affymetrix GeneTitan instrument (Affymetrix, Santa Clara, CA).
Description
12_EDL13_Ctrl
Data processing
Expression estimates were calculated applying the RMA algorithm in the Bioconductor library 'Oligo' (v1.44.0).
