|
| Status |
Public on Jun 01, 2019 |
| Title |
Vehicle replicate 1, GFP+ cells from 3 mice [Kentucky assay: undecanal] |
| Sample type |
RNA |
| |
|
| Source name |
Olfactory mucosa; Vehicle-stimulated
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
tissue: Olfactory mucosa
agent: Vehicle
cell type: FACS sample, GFP-
|
| Treatment protocol |
After 26 hrs, half the mice were intermittently stimulated with vehicle (filtered air) or odorant for 10 sec every 20 min.
|
| Growth protocol |
24 mice of both sexes, ages 7 - 12 weeks, were placed individuallly in clean air chambers for 40 hrs under air flow of 3.1L/min
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Mice were euthanized, olfactory mucosa were dissected, dissociated, and subjected to FACS.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA was prepared according to the standard Affymetrix protocol from 50 - 100 ng of total RNA.
|
| |
|
| Hybridization protocol |
after fragmentation cRNA was hybridized for 16 hr at 45C on Clariom S Arrays. These were washed and stained in an Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using an Affymetrix GCS3000 7G Scanner
|
| Description |
mRNA abundance data
|
| Data processing |
The data were analyzed with Affymetrix Gene Expression Console software using default analysis settings and global scaling as normalization method.
|
| |
|
| Submission date |
Dec 13, 2018 |
| Last update date |
Jun 02, 2019 |
| Contact name |
Timothy S McClintock |
| E-mail(s) |
mcclint@uky.edu
|
| Phone |
8599403364
|
| Organization name |
University of kentucky
|
| Department |
Physiology
|
| Lab |
Timothy McClintock
|
| Street address |
508 Medical Science Buildi
|
| City |
Lexington |
| State/province |
Kentucky |
| ZIP/Postal code |
40536 |
| Country |
USA |
| |
|
| Platform ID |
GPL23038 |
| Series (1) |
|
