|
| Status |
Public on Apr 23, 2020 |
| Title |
12_SCtoLW_LW_male6 |
| Sample type |
RNA |
| |
|
| Source name |
LW conditions, whole brain, replicate 6
|
| Organism |
Oryzias latipes |
| Characteristics |
tissue: whole brain
gender: male
treatment: LW (long-day and warm temperature conditions: 14 h light/10 h dark and 26 °C)
|
| Treatment protocol |
Adult male medaka kept under SC conditions either reimained in SC or were transferred into LW conditions. Two weeks after transfer, whole brains were collected in the middle of the light phase.
|
| Growth protocol |
Medaka fish (Oryzias latipes) were obtained from a local dealer and kept outdoors.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was prepared from each fish using an RNeasy tissue kit (QIAGEN).
|
| Label |
Cy3
|
| Label protocol |
cDNA synthesis and cRNA labeling reactions were performed with the Low Input Quick Amp Labeling Kit (Agilent Technologies).
|
| |
|
| Hybridization protocol |
Labeled cRNA was purified with RNeasy mini spin columns (QIAGEN) and hybridized using the Gene Expression Hybridization Kit (Agilent Technologies).
|
| Scan protocol |
After washing with Gene Expression Wash Buffer (Agilent Technologies), the slide glass was scanned on a Microarray Scanner (Agilent Technologies).
|
| Description |
Gene expression of whole brain under LW conditions
|
| Data processing |
Microarray data were analyzed using the GeneSpring software (Agilent Technologies).
|
| |
|
| Submission date |
Jan 11, 2019 |
| Last update date |
Apr 25, 2020 |
| Contact name |
Takashi Yoshimura |
| E-mail(s) |
takashiy@agr.nagoya-u.ac.jp
|
| Organization name |
Nagoya University
|
| Street address |
Furo-cho, Chikusa-ku
|
| City |
Nagoya |
| ZIP/Postal code |
4648601 |
| Country |
Japan |
| |
|
| Platform ID |
GPL23001 |
| Series (2) |
| GSE124973 |
Seasonal changes of gene expression of whole brain in Medaka fish (Oryzias latipes) SC |
| GSE124988 |
Seasonal transcriptional landscape of medaka brain |
|
