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Sample GSM357343 Query DataSets for GSM357343
Status Public on Jan 07, 2009
Title PRC SiRNA time 48 of 20% serum induction replicat 3
Sample type RNA
 
Channel 1
Source name XTC.UC1 cells
Organism Homo sapiens
Characteristics XTC.UC1 cells : PRC SiRNA time 48 of serum induction
Extracted molecule total RNA
Extraction protocol Total RNA extracted using RNeasy columns following manufacturer's instructions
Label Cy5
Label protocol protocol available on http://cardioserve.nantes.inserm.fr/ptf-puce/IMG/pdf/Pr_ration_Cibles-P1.2_Ampli_Marq_Ambion.pdf
 
Channel 2
Source name XTC.UC1 cells
Organism Homo sapiens
Characteristics total RNA from pooled XTC
Extracted molecule total RNA
Extraction protocol Total RNA extracted using RNeasy columns following manufacturer's instructions
Label Cy3
Label protocol protocol available on http://cardioserve.nantes.inserm.fr/ptf-puce/IMG/pdf/Pr_ration_Cibles-P1.2_Ampli_Marq_Ambion.pdf
 
 
Hybridization protocol Protocol available on http://cardioserve.nantes.inserm.fr/ptf-puce/IMG/pdf/Microarray_-P2.2__Hybridations_lames_epoxy_Lucidea-2.pdf
Scan protocol Scanned on ScanArray express II (Packard) and using genepix 4.0 software (Axon)
Description PRC SIRNA time 48
Data processing LOWESS normalized, background subtracted data obtained from log2 of processed Red signal/processed Green signal.
 
Submission date Jan 05, 2009
Last update date Jan 06, 2009
Contact name frederique savagner
E-mail(s) frederique.savagner@univ-angers.fr
Phone +33241353314
Fax +33241354017
Organization name Inserm
Department U694
Street address 4 rue larrey
City angers
ZIP/Postal code 49033
Country France
 
Platform ID GPL7674
Series (1)
GSE14282 PRC SiRNA vs scramble transfection in XTC.UC1 cells

Data table header descriptions
ID_REF
VALUE normalized log2 ratio (Cy5/Cy3) representing test/reference

Data table
ID_REF VALUE
1 1.050532619
2 1.000963133
3 0.837742992
4 1.481136574
5 1.214027888
6 1.173478492
7 1.188713839
8 0.94153753
9 0.887781999
10 1.454222405
11 0.956550239
12 0.9568388
13 1
14 0.89470897
15 1.001811682
16 0.628869882
17 0.854523631
18 0.763092066
19 0.917287493
20 0.837241362

Total number of rows: 20152

Table truncated, full table size 340 Kbytes.




Supplementary file Size Download File type/resource
GSM357343.gpr.gz 1.8 Mb (ftp)(http) GPR
Processed data included within Sample table
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