NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM357457 Query DataSets for GSM357457
Status Public on May 27, 2009
Title Kidney_M4
Sample type RNA
 
Source name irradiated mouse kidney_0.65 μGy/min (420 mGy)
Organism Mus musculus
Characteristics Male specific pathogen free C57BL/6J
Biomaterial provider CLEA Japan, Inc.
Treatment protocol Irradiation with 137Cs γ-rays was carried out in a facility at the Institute for Environmental Sciences for 485 days (22 h/day) at dose-rates of 0.032 μGy/min, 0.65 μGy/min, and 13 μGy/min. The total dose after irradiation at these dose-rates was 21 mGy, 420 mGy, and 8000 mGy. We examined 12 mice (3 per each dose-rate condition, including the control). Mice were euthanized immediately after the termination of irradiation, and the kidney and testis were removed and stored at -80°C in RNAlater (Ambion).
Extracted molecule total RNA
Extraction protocol RNAlater and RNeasy Mini Kit and RNase-Free DNase Set (Qiagen, Valencia, CA)
Label biotin
Label protocol Illumina RNA Amplification Kit (Label with biotin-UTP directly)
 
Hybridization protocol Illumina Gene Expression system Hyb E1 and Block Buffer kit,
Wash E1BC Buffer kit and High Temp Wash Buffer kit
Scan protocol Standard of BeadStation 500GX-WG system (Illumina)
Description Sample identifier: K-400-4
Data processing Rank-invariant normalization (http://www.mged.org/normalization)
 
Submission date Jan 05, 2009
Last update date May 27, 2009
Contact name Mitsuru Nenoi
Fax 81-43-255-6497
URL http://www.nirs.go.jp/ENG/research/radiation_protection/05.shtml
Organization name National institute of Radiological Sciences
Department Research Center for Radiation Protection
Lab Radiation Effect Mechanisms Research Group
Street address 4-9-1, Anagawa, Inage-ku
City Chiba
ZIP/Postal code 263-8555
Country Japan
 
Platform ID GPL4234
Series (1)
GSE14290 Microarray analysis of gene expression in the kidneys and testes of mice after low-dose-rate irradiation

Data table header descriptions
ID_REF
VALUE normalized signal

Data table
ID_REF VALUE
102450270 14.358
104200035 -18.7012
100730138 9.51729
102320427 -20.9998
102630735 -12.2101
100450435 7.95105
101230594 -28.4408
100780541 28.9761
104200184 -0.166107
105360286 -11.2444
103060411 13.6815
100630193 -11.7456
105270286 -28.9966
101690685 -25.978
100060180 -8.78069
107100563 -20.7578
100450451 -13.2862
100610092 -22.0099
104810452 46398.7
6840019 13819.3

Total number of rows: 46116

Table truncated, full table size 773 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap