|
Status |
Public on Jul 16, 2019 |
Title |
5293 GMP: 10 wk GMP, MLL-ENL (x2), No dox |
Sample type |
SRA |
|
|
Source name |
bone marrow
|
Organism |
Mus musculus |
Characteristics |
tissue: bone marrow age: 10 weeks genotype: MLL-ENL cell type: granulocyte-monocyte progenitor
|
Extracted molecule |
total RNA |
Extraction protocol |
Qiagen RNAeasy micro plus Clontech SMRTr
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina HiSeq 3000 |
|
|
Description |
fetal induction
|
Data processing |
The resulting sequencing reads were demultiplexed by index with a custom Python demultiplexing script and further demultiplexed by barcode with a custom Perl demultiplexing script. The RNA-Seq reads were then aligned to the Mus musculus Ensembl release 76 top-level assembly with STAR version 3.0.4.b. Gene counts were derived from the number of uniquely aligned unambiguous reads by Subread:featureCount version 1.4.5. Sailfish version 0.6.3. was used to produce isoform estimated counts. Genome_build: Ensemble R76 Supplementary_files_format_and_content: tab-delimited text files include read count values for each Sample
|
|
|
Submission date |
Jan 24, 2019 |
Last update date |
Jul 16, 2019 |
Contact name |
Jeffery Magee |
E-mail(s) |
magee_j@kids.wustl.edu
|
Organization name |
Washington University School of Medicine
|
Department |
Internal Medicine - Cardiovascular Division
|
Street address |
660 S Euclid Ave.
|
City |
St Louis |
State/province |
Missouri |
ZIP/Postal code |
63110 |
Country |
USA |
|
|
Platform ID |
GPL21493 |
Series (1) |
GSE125549 |
The efficiency of MLL-ENL driven leukemia initiation changes with age and peaks during neonatal development |
|
Relations |
BioSample |
SAMN10798041 |
SRA |
SRX5286004 |