|
Status |
Public on Jul 31, 2020 |
Title |
Ependymoma_recurrent (246-2) |
Sample type |
RNA |
|
|
Source name |
246-2
|
Organism |
Homo sapiens |
Characteristics |
tissue: brain tumor (ependymoma) tumor type: recurrent
|
Extracted molecule |
total RNA |
Extraction protocol |
[collection protocol] Tumor samples were snap frozen in liquid nitrogen or transferred to RNAlater immediately after surgical removal as part of patient care. All samples were collected with appropriate IRB approval (COM-IRB#95-500 and 09-0906 ) RNeasy (Qiagen) extraction of total RNA was performed according to the manufacturer's instructions.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix).
|
|
|
Hybridization protocol |
Following fragmentation, 11.5 ug of cRNA were hybridized for 16 hr at 45C on GeneChips. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
|
Scan protocol |
GeneChips were scanned using the Affymetrix Gene Chip Scanner 7G.
|
Data processing |
Microarray .CEL file data was background corrected and normalized using the gcRMA algorithm, resulting in log 2 expression values.
|
|
|
Submission date |
Jan 29, 2019 |
Last update date |
Jul 31, 2020 |
Contact name |
Andrew M Donson |
E-mail(s) |
andrew.donson@cuanschutz.edu
|
Phone |
303 724 4012
|
Organization name |
CU Anschutz Medical Campus
|
Department |
Pediatrics
|
Lab |
RC1 North, P18-4401M
|
Street address |
12800 E 19th Ave
|
City |
Aurora |
State/province |
CO |
ZIP/Postal code |
80010 |
Country |
USA |
|
|
Platform ID |
GPL570 |
Series (2) |
GSE125861 |
Ependymoma subpopulation lineages underlie clinical classification and outcome (microarray data set) |
GSE126025 |
Ependymoma subpopulation lineages underlie clinical classification and outcome |
|
Relations |
Reanalysis of |
GSM1620224 |