|
| Status |
Public on Oct 29, 2019 |
| Title |
Patient 19, glomerulus |
| Sample type |
RNA |
| |
|
| Source name |
Patient 19, glomerular tissue
|
| Organism |
Homo sapiens |
| Characteristics |
subject id: Patient 19
class: 4
subclass: 1
tissue: glomerular tissue
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from micro-dissected glomerular and tubulointerstitial comparments by silica gel columns and diluted in 30 μL RNase-free water.
|
| Label |
biotin
|
| Label protocol |
Manufacturer's recommended protocols as followed by the TCAG Facility at the Hospital for Sick Kid's in Toronto, Canada
|
| |
|
| Hybridization protocol |
Manufacturer's recommended protocols as followed by the TCAG Facility at the Hospital for Sick Kid's in Toronto, Canada
|
| Scan protocol |
Manufacturer's recommended protocols as followed by the TCAG Facility at the Hospital for Sick Kid's in Toronto, Canada
|
| Data processing |
Raw array data (CEL files) were loaded into the R statistical environment (v3.3.1) using the affy package (v1.48.0) of the BioConductor library. Data was investigated for distributional homogeneity, then pre-processed using the RMA algorithm. An updated Probe to Entrez Gene ID map, hta20hsentrezgcdf (v20.0.0), was used for annotation.
|
| |
|
| Submission date |
Mar 04, 2019 |
| Last update date |
Oct 29, 2019 |
| Contact name |
Stephenie Prokopec |
| E-mail(s) |
stephenie.prokopec@oicr.on.ca
|
| Organization name |
Ontario Institute for Cancer Research
|
| Street address |
661 University Avenue, Suite 510
|
| City |
Toronto |
| State/province |
Ontario |
| ZIP/Postal code |
M5G 0A3 |
| Country |
Canada |
| |
|
| Platform ID |
GPL24299 |
| Series (1) |
| GSE127797 |
The Molecular Phenotype of Lupus Nephritis Histological Lesions |
|
