|
Status |
Public on Aug 19, 2020 |
Title |
Fabp26KO-F-2: Fabp2&6DKO_female_430A_replicate2 |
Sample type |
RNA |
|
|
Source name |
small intestine from Fabp2 and Fabp6-simultaneously deleted C57BL/6J mouse
|
Organism |
Mus musculus |
Characteristics |
tissue: small intestine age: 10-week old genotype: Fabp2-/-;Fabp6-/- C57BL/6J
|
Treatment protocol |
No treatment
|
Growth protocol |
Mice were housed in a sterile and temperature controlled environment and fed a standard lab diet (Purina 5001).
|
Extracted molecule |
total RNA |
Extraction protocol |
The small intestine, starting from the base of the stomach and ending just before the cecum, was excised and then flushed with ice-cold saline prior to tissue homogenization. RNA was extracted from homogenates using Trizol (Invitrogen) and assessed for integrity (RIN>7) prior to use in microarray analysis.
|
Label |
Cy3
|
Label protocol |
Fluorescently labeled cDNA probes for each RNA pool was prepared following the procedures specified by the manufacturer.
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|
|
Hybridization protocol |
cDNA was hybridized to Affymetrix 430A and 430B chips following the procedures specified by the manufacturer.
|
Scan protocol |
Signals were acquired using the Affymetrix GeneChip Scanner Model 3000.
|
Description |
gene expression of the small intestine
|
Data processing |
The data were processed and analyzed using the R statistical environment and Bioconductor software (www.bioconductor.org) and to log2 scale using the RMA algorithm with background correction
|
|
|
Submission date |
Mar 26, 2019 |
Last update date |
Aug 19, 2020 |
Contact name |
Luis B Agellon |
E-mail(s) |
luis.agellon@mcgill.ca
|
Phone |
1-514-398-7862
|
Organization name |
McGill University
|
Department |
School of Human Nutrition
|
Street address |
21111 Lakeshore Road
|
City |
Ste-Anne-de-Bellevue |
State/province |
QC |
ZIP/Postal code |
H9X 3V9 |
Country |
Canada |
|
|
Platform ID |
GPL339 |
Series (1) |
GSE128862 |
Sexually dimorphic characteristics of the small intestinal transcriptome of Fabp-gene ablated mice |
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