|
| Status |
Public on Jun 06, 2019 |
| Title |
SJDMNORM011377_G1-6WKB3-3 |
| Sample type |
SRA |
| |
|
| Source name |
skeletal muscles
|
| Organism |
Drosophila melanogaster |
| Characteristics |
strain: B3 parental
tissue: Skeletal muscles
age: Old (6 weeks old)
|
| Treatment protocol |
NA, genotype based.
|
| Growth protocol |
NA
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol
RNA libraries were prepared for sequencing using standard Illumina protocols.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Description |
B3 parental old (6 weeks old)
RNA-Seq Stranded mRNA
|
| Data processing |
Illumina Casava1.7 software used for basecalling.
Sequenced reads were trimmed for adaptor sequence, and then mapped to dm3 whole genome using an internal pipeline that employs bwa and STAR.
HTSeq was used to extract raw counts.
Genome_build: dm3 (Release 5)
Supplementary_files_format_and_content: *_merged.txt: Gene symbols and raw count values in 2-column tab-delimited txt file, one for each sample.
|
| |
|
| Submission date |
Apr 16, 2019 |
| Last update date |
Jun 06, 2019 |
| Contact name |
David Finkelstein |
| E-mail(s) |
david.finkelstein@stjude.org
|
| Phone |
9014953931
|
| Organization name |
St Jude Children's Research Hospital
|
| Department |
Computational Biology
|
| Street address |
332 N. Lauderdale St.
|
| City |
Memphis |
| State/province |
TN |
| ZIP/Postal code |
38105 |
| Country |
USA |
| |
|
| Platform ID |
GPL13304 |
| Series (1) |
| GSE129922 |
Circadian gene variants and the skeletal muscle circadian clock contribute to the evolutionary divergence in longevity across Drosophila populations |
|
| Relations |
| BioSample |
SAMN11442940 |
| SRA |
SRX5700885 |
