|
| Status |
Public on Jul 15, 2019 |
| Title |
dEEDcl72 |
| Sample type |
SRA |
| |
|
| Source name |
osteosarcoma-derived cell line
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: ipNF05.5
genotype: EED KO
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA Picopure Extraction Kite KIT0204, Life Tech
SMART-Seq® v4 Ultra® Low Input RNA Kit for Sequencing, Ref-634888
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Data processing |
Raw reads were trimmed for adapters with cutadapt (1.12) using the Trim Galore! (0.4.4) wrapper (default settings) and subsequently mapped to the complete rRNA sequence with Bowtie2 (2.2.9).
Reads that did not map to rRNA were then mapped with STAR (2.5.3a) to the full reference genome.
Gene counts were generated using STAR --quant_mode.
Genome_build: hg19 (ipNF05.5 cells)
Genome_build: GRCm38/mm10 (spermatogonia and oocytes)
Supplementary_files_format_and_content: gene counts
|
| |
|
| Submission date |
Apr 23, 2019 |
| Last update date |
Jul 15, 2019 |
| Contact name |
Nicolas Servant |
| E-mail(s) |
Nicolas.Servant@curie.fr
|
| Organization name |
Institut Curie
|
| Street address |
26 rue d'ulm
|
| City |
Paris Cedex 05 |
| ZIP/Postal code |
75248 |
| Country |
France |
| |
|
| Platform ID |
GPL16791 |
| Series (2) |
| GSE130229 |
EZHIP constrains Polycomb Repressive Complex 2 activity in germ cells (RNA-Seq) |
| GSE130231 |
EZHIP constrains Polycomb Repressive Complex 2 activity in germ cells |
|
| Relations |
| BioSample |
SAMN11484310 |
| SRA |
SRX5726991 |
