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Sample GSM3942852 Query DataSets for GSM3942852
Status Public on Jan 11, 2021
Title 3632
Sample type RNA
 
Source name blood
Organism Homo sapiens
Characteristics age: 21
gender: male
disease state: noninfectious
tissue: blood
Treatment protocol PAXgene blood RNA tubes were left at room temperaturee to defrost (approx. 2 hours) while mixing by inversion.
Growth protocol Whole blood was collected in PAXgene blood RNA tubes, mixed by inversion 10X and stored at -80C.
Extracted molecule total RNA
Extraction protocol Total RNA was isolated in accordance with the PAXgene blood miRNA isolation (QIAGEN) procedure using the QIAcube instrument.
Label biotin
Label protocol 100ng total RNA was labeled using standard GeneChip buffers and reagents (Affymetrix)
 
Hybridization protocol Standard GeneChip Hybridization, Wash, and Stain Kit (Affymetrix).
Scan protocol HTA 2.0 chips were scanned at the Cologne Center for Genomics, Cologne, Germany.
Description Gene expression data from adults
Data processing Raw data scans (.CEL files) were read into the R language and environment for statistical computing (version 3.5.0; R Foundation for Statistical Computing, Vienna, Austria; http://www.R-project.org/). Pre-processing and quality control was performed by using the Oligo package. The core array data were background corrected by Robust Multi-array Average (RMA) and quantiles-normalized.
 
Submission date Jul 16, 2019
Last update date Jan 11, 2021
Contact name Brendon Scicluna
E-mail(s) brendon.scicluna@um.edu.mt
Organization name University of Malta
Street address Msida campus
City Msida
ZIP/Postal code MSD2020
Country Malta
 
Platform ID GPL17586
Series (2)
GSE134347 Protein-coding and non-coding RNA expression in whole blood leukocytes of healthy subjects and critically-ill patients with sepsis or non-infectious etiology.
GSE134364 Protein-coding and non-coding RNA landscape in critically ill patients with sepsis

Data table header descriptions
ID_REF
VALUE log2

Data table
ID_REF VALUE
2824546_st 9.704978887
2824549_st 8.962626848
2824551_st 8.730090269
2824554_st 8.591900119
2827992_st 9.483979481
2827995_st 8.845661167
2827996_st 9.098596545
2828010_st 6.022241287
2828012_st 6.292357293
2835442_st 6.282349468
2835447_st 5.707818724
2835453_st 3.946645176
2835456_st 9.864194207
2835459_st 8.224026146
2835461_st 4.487378498
2839509_st 9.300004295
2839511_st 7.468270896
2839513_st 8.67112914
2839515_st 6.495413469
2839517_st 9.082758258

Total number of rows: 70523

Table truncated, full table size 1930 Kbytes.




Supplementary file Size Download File type/resource
GSM3942852_COM_P4_3632_190416_HTA-2_0_.CEL.gz 23.1 Mb (ftp)(http) CEL
Processed data included within Sample table

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