|
Status |
Public on Jan 11, 2021 |
Title |
hv181 |
Sample type |
RNA |
|
|
Source name |
blood
|
Organism |
Homo sapiens |
Characteristics |
age: 60 gender: female disease state: healthy tissue: blood
|
Treatment protocol |
PAXgene blood RNA tubes were left at room temperaturee to defrost (approx. 2 hours) while mixing by inversion.
|
Growth protocol |
Whole blood was collected in PAXgene blood RNA tubes, mixed by inversion 10X and stored at -80C.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was isolated in accordance with the PAXgene blood miRNA isolation (QIAGEN) procedure using the QIAcube instrument.
|
Label |
biotin
|
Label protocol |
100ng total RNA was labeled using standard GeneChip buffers and reagents (Affymetrix)
|
|
|
Hybridization protocol |
Standard GeneChip Hybridization, Wash, and Stain Kit (Affymetrix).
|
Scan protocol |
HTA 2.0 chips were scanned at the Cologne Center for Genomics, Cologne, Germany.
|
Description |
Gene expression data from adults
|
Data processing |
Raw data scans (.CEL files) were read into the R language and environment for statistical computing (version 3.5.0; R Foundation for Statistical Computing, Vienna, Austria; http://www.R-project.org/). Pre-processing and quality control was performed by using the Oligo package. The core array data were background corrected by Robust Multi-array Average (RMA) and quantiles-normalized.
|
|
|
Submission date |
Jul 16, 2019 |
Last update date |
Jan 11, 2021 |
Contact name |
Brendon Scicluna |
E-mail(s) |
brendon.scicluna@um.edu.mt
|
Organization name |
University of Malta
|
Street address |
Msida campus
|
City |
Msida |
ZIP/Postal code |
MSD2020 |
Country |
Malta |
|
|
Platform ID |
GPL17586 |
Series (2) |
GSE134347 |
Protein-coding and non-coding RNA expression in whole blood leukocytes of healthy subjects and critically-ill patients with sepsis or non-infectious etiology. |
GSE134364 |
Protein-coding and non-coding RNA landscape in critically ill patients with sepsis |
|