|
| Status |
Public on Jan 11, 2021 |
| Title |
COM_P6_100232_080616 |
| Sample type |
RNA |
| |
|
| Source name |
blood
|
| Organism |
Homo sapiens |
| Characteristics |
treatment: endo_2h
age: 25
gender: male
treatment: endotoxemia
tissue: blood
|
| Treatment protocol |
PAXgene blood RNA tubes were left at room temperaturee to defrost (approx. 2 hours) while mixing by inversion.
|
| Growth protocol |
Whole blood was collected in PAXgene blood RNA tubes, mixed by inversion 10X and stored at -80C.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated in accordance with the PAXgene blood miRNA isolation (QIAGEN) procedure using the QIAcube instrument.
|
| Label |
biotin
|
| Label protocol |
100ng total RNA was labeled using standard GeneChip buffers and reagents (Affymetrix)
|
| |
|
| Hybridization protocol |
Standard GeneChip Hybridization, Wash, and Stain Kit (Affymetrix).
|
| Scan protocol |
HTA 2.0 chips were scanned at the Cologne Center for Genomics, Cologne, Germany.
|
| Description |
Gene expression data from adults
|
| Data processing |
Raw data scans (.CEL files) were read into the R language and environment for statistical computing (version 3.5.0; R Foundation for Statistical Computing, Vienna, Austria; http://www.R-project.org/). Pre-processing and quality control was performed by using the Oligo package. The core array data were background corrected by Robust Multi-array Average (RMA) and quantiles-normalized.
|
| |
|
| Submission date |
Jul 16, 2019 |
| Last update date |
Jan 11, 2021 |
| Contact name |
Brendon Scicluna |
| E-mail(s) |
brendon.scicluna@um.edu.mt
|
| Organization name |
University of Malta
|
| Street address |
Msida campus
|
| City |
Msida |
| ZIP/Postal code |
MSD2020 |
| Country |
Malta |
| |
|
| Platform ID |
GPL17586 |
| Series (2) |
| GSE134356 |
Protein-coding and non-coding RNA expression in whole blood leukocytes during experimental human endotoxemia. |
| GSE134364 |
Protein-coding and non-coding RNA landscape in critically ill patients with sepsis |
|
