|
Status |
Public on Jan 11, 2021 |
Title |
100114 |
Sample type |
RNA |
|
|
Source name |
blood
|
Organism |
Homo sapiens |
Characteristics |
age: 23 gender: male disease state: patient treatment: none tissue: blood
|
Treatment protocol |
PAXgene blood RNA tubes were left at room temperaturee to defrost (approx. 2 hours) while mixing by inversion.
|
Growth protocol |
Whole blood was collected in PAXgene blood RNA tubes, mixed by inversion 10X and stored at -80C.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was isolated in accordance with the PAXgene blood miRNA isolation (QIAGEN) procedure using the QIAcube instrument.
|
Label |
biotin
|
Label protocol |
100ng total RNA was labeled using standard GeneChip buffers and reagents (Affymetrix)
|
|
|
Hybridization protocol |
Standard GeneChip Hybridization, Wash, and Stain Kit (Affymetrix).
|
Scan protocol |
miRNA-4_1 chips were scanned at the Cologne Center for Genomics, Cologne, Germany.
|
Description |
Gene expression data from adults
|
Data processing |
Pre-processing, normalization, probe summarization, and data quality control were executed in Expression Console Software (Affymetrix). Robust Multi-array Average (RMA) normalization and DABG probe-level detection were specified. All other parameters were default.
|
|
|
Submission date |
Jul 16, 2019 |
Last update date |
Jan 11, 2021 |
Contact name |
Brendon Scicluna |
E-mail(s) |
brendon.scicluna@um.edu.mt
|
Organization name |
University of Malta
|
Street address |
Msida campus
|
City |
Msida |
ZIP/Postal code |
MSD2020 |
Country |
Malta |
|
|
Platform ID |
GPL21572 |
Series (2) |
GSE134358 |
Small non-coding RNA expression in whole blood leukocytes of healthy subjects, critically-ill patients with sepsis or non-infectious etiology and human endotoxemia |
GSE134364 |
Protein-coding and non-coding RNA landscape in critically ill patients with sepsis |
|