|
| Status |
Public on Jan 11, 2021 |
| Title |
3910077 |
| Sample type |
RNA |
| |
|
| Source name |
blood
|
| Organism |
Homo sapiens |
| Characteristics |
age: 49
gender: male
disease state: sepsis
treatment: none
tissue: blood
|
| Treatment protocol |
PAXgene blood RNA tubes were left at room temperaturee to defrost (approx. 2 hours) while mixing by inversion.
|
| Growth protocol |
Whole blood was collected in PAXgene blood RNA tubes, mixed by inversion 10X and stored at -80C.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated in accordance with the PAXgene blood miRNA isolation (QIAGEN) procedure using the QIAcube instrument.
|
| Label |
biotin
|
| Label protocol |
100ng total RNA was labeled using standard GeneChip buffers and reagents (Affymetrix)
|
| |
|
| Hybridization protocol |
Standard GeneChip Hybridization, Wash, and Stain Kit (Affymetrix).
|
| Scan protocol |
miRNA-4_1 chips were scanned at the Cologne Center for Genomics, Cologne, Germany.
|
| Description |
Gene expression data from adults
|
| Data processing |
Pre-processing, normalization, probe summarization, and data quality control were executed in Expression Console Software (Affymetrix). Robust Multi-array Average (RMA) normalization and DABG probe-level detection were specified. All other parameters were default.
|
| |
|
| Submission date |
Jul 16, 2019 |
| Last update date |
Jan 11, 2021 |
| Contact name |
Brendon Scicluna |
| E-mail(s) |
brendon.scicluna@um.edu.mt
|
| Organization name |
University of Malta
|
| Street address |
Msida campus
|
| City |
Msida |
| ZIP/Postal code |
MSD2020 |
| Country |
Malta |
| |
|
| Platform ID |
GPL21572 |
| Series (2) |
| GSE134358 |
Small non-coding RNA expression in whole blood leukocytes of healthy subjects, critically-ill patients with sepsis or non-infectious etiology and human endotoxemia |
| GSE134364 |
Protein-coding and non-coding RNA landscape in critically ill patients with sepsis |
|
