|
| Status |
Public on Aug 22, 2020 |
| Title |
control, NCI/ADR-RES cells rep 1 |
| Sample type |
RNA |
| |
|
| Source name |
RNA from control, NCI/ADR-RES cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: NCI/ADR-RES
gender: female
|
| Treatment protocol |
Cells were left untreated or treated with DETNO, ADR, and combinations of DETNO and ADR for 24 hrs.
|
| Growth protocol |
NCI/ADR-RES tumor cells were grown in 1640 RPMI media containing 10 % FBS to 70-75% confluency.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using Triazol method.
|
| Label |
biotin
|
| Label protocol |
One hundred nanograms (100 ng) of total RNA were amplified and labeled as directed using the Affymetrix WT Plus Reagent Kit (WT Plus Kit).
|
| |
|
| Hybridization protocol |
Five and one-half micrograms (5.5 μg) of amplified biotin-cDNAs were fragmented and hybridized to each array for 16 hours at 45°C in a rotating hybridization oven Array slides were stained with streptavidin/phycoerythrin utilizing a double-antibody staining procedure and then washed for antibody amplification per the GeneChip Hybridization, Wash and Stain Kit and user manual following protocol FS450-0001.
|
| Scan protocol |
Arrays were scanned in an Affymetrix Scanner 3000.
|
| Description |
Gene expression from control, NCI/ADR-RES cells
|
| Data processing |
Data was obtained using TAC Software.
|
| |
|
| Submission date |
Aug 22, 2019 |
| Last update date |
Aug 23, 2020 |
| Contact name |
NIEHS Microarray Core |
| E-mail(s) |
microarray@niehs.nih.gov, liuliw@niehs.nih.gov
|
| Organization name |
NIEHS
|
| Department |
DIR
|
| Lab |
Microarray Core
|
| Street address |
111 T.W. Alexander Drive
|
| City |
RTP |
| State/province |
NC |
| ZIP/Postal code |
27709 |
| Country |
USA |
| |
|
| Platform ID |
GPL17586 |
| Series (1) |
| GSE136201 |
Effect of nitric oxide on the reversal of adriamycin resistance in NCI/ADR-RES tumor cells |
|
