|
| Status |
Public on Dec 15, 2009 |
| Title |
3 hour old early-schistosomula treated with human TNF-alpha bio rep 1 tech rep 2 cy3 |
| Sample type |
RNA |
| |
|
| Source name |
3 hour old early-schistosomula
|
| Organism |
Schistosoma mansoni |
| Characteristics |
stage: cercariae-to-schistosomula
|
| Treatment protocol |
The Schistosoma mansoni just 3 h after cercariae-to-schistosomula in vitro transformation were incubated in vitro (37 oC, 5% CO2 ) with Tris-HCL (10nM) pH 8,8 during 1h.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was obtained using Trizol reagent (Invitrogen) according to the manufacturer's instructions. The RNA was quantified using the Nanodrop ND-1000 UV/Vis spectrophotometer and RNA integrity was checked by electrophoresis with an Agilent 2100 Bioanalyzer. Total RNA was treated with DNAse I (QIAGEN) and subsequently purified using RNA easy mini kit
|
| Label |
Cy3
|
| Label protocol |
Three hundred nanograms of total RNA from each sample were amplified using the Linear RNA amplification and labeling kit (Agilent Technologies) according to manufacturer´s instructions. Eight hundred and twenty five nanograms Cy3 labeled cRNA was used to hibridization.
|
| |
|
| Hybridization protocol |
The hybridization solution and procedure were prepared according to manufacturer´s instructions.
|
| Scan protocol |
Slides were air dried and scanned using a microarray dual channel laser scanner (GenePix 4000B, Molecular Devices, USA) 100 % laser power and PMT levels were adjusted in order to obtain similar average intensities of red and green signal.
|
| Description |
No additional information
|
| Data processing |
Data was extracted from images using the Feature Extraction software (Agilent Technologies). Spots with low intensities were filtered out according to criteria established by Feature Extraction software (IsPosAndSig collun). Normalization was perfomed using the Quantiles algorithm.
|
| |
|
| Submission date |
May 27, 2009 |
| Last update date |
Mar 11, 2011 |
| Contact name |
Katia Cristina Pereira Oliveira |
| Organization name |
Instituto Adolfo Lutz
|
| Department |
Centro de Parasitologia e Micologia
|
| Lab |
Nucleo de Enterparasitas
|
| Street address |
Av Prof lineu prestes 748
|
| City |
São Paulo |
| State/province |
O |
| ZIP/Postal code |
05508000 |
| Country |
Brazil |
| |
|
| Platform ID |
GPL8606 |
| Series (1) |
| GSE16260 |
Effect of human TNF-alpha on schistosomulum stage of Schistosoma mansoni gene expression profile |
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